Han, Woo-Cheul Byun, Sun-Ho Kim, Mi-Hyun Sohn, Eun Hwa Lim, Jung Dae Um, Byung Hun Kim, Chul Ho Kang, Soon Ah Jang, Ki-Hyo 2024-01-20T20:33:34Z 2024-01-20T20:33:34Z 2021-09-05 2009-10 1017-7825 https://pubs.kist.re.kr/handle/201004/132103 Lactosucrose (4(G)-beta-D-galactosylsucrose) is in oligosaccharide consisting of galactose, glucose, and fructose. In this study we prepared lactosucrose from lactose and sucrose using a levansucrase derived from Zymomonas mobilis. Optimum conditions for lactosucrose formation were 23 degrees C, pH 7.0, 18.0% (w/v) lactose monohydrate, and 18% (w/v) sucrose as substrates, and 1 unit of enzyme/ml of reaction mixture. Under these conditions, the lactosucrose conversion efficiency was 28.5%. The product was purified and confirmed to he O-beta-D-galactopyranosyl-(1 -> 4)-O-beta-D-glucopyranosyl-(1 -> 2)-beta-D-fructofuranoside, or lactosucrose. A mixed-enzyme system containing a levansucrase and a glucose oxidase was applied in order to increase the efficiency of lactose and sucrose conversion to lactosucrose, which rose to 43.2% as a result. English KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY ESCHERICHIA-COLI BETA-FRUCTOFURANOSIDASE ENZYMATIC-SYNTHESIS OLIGOSACCHARIDES SECRETION INULIN LEVAN Production of Lactosucrose from Sucrose and Lactose by a Levansucrase from Zymomonas mobilis Article 10.4014/jmb.0901.045 1 JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.19, no.10, pp.1153 - 1160 JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 19 10 1153 1160 scie scopus kci 000271230600013 Biotechnology & Applied Microbiology Microbiology Biotechnology & Applied Microbiology Microbiology Article ESCHERICHIA-COLI BETA-FRUCTOFURANOSIDASE ENZYMATIC-SYNTHESIS OLIGOSACCHARIDES SECRETION INULIN LEVAN Glucose oxidase lactosucrose levansucrase Zymomonas mobilis