Lee, J.Y. Song, D.-G. Jung, S.H. Kim, J.H. Ahn, S.Y. Nho, C.W. Pan, C.-H. 2024-01-20T20:34:22Z 2024-01-20T20:34:22Z 2021-09-02 2009-10 0253-3073 https://pubs.kist.re.kr/handle/201004/132144 We examined ethanol extracts prepared from 23 Korean local plants obtained in Pyeongchang, Gangwon-do for their inhibitory effects on recombinant human AKR 1 B10 (rhAKR1B10) in vitro. To do this, rhAKR1B10 was first expressed in E. coli as a biological active form and purified by using Ni-affinity chromatography followed by gel permeation chromatography. Then, rhAKR1B10 was used for screening out 23 Korean local plant extracts having an inhibitory activity against itself. Among them, six extracts showed more than 50% inhibition of rhAKR1B10 activity at the concentration of 10 μg/ml. Especially, the extracts of Ligularia fischeri var. spiciformis Nakai and Rhus trichocarpa Miq. were the most potent because their IC50 values were 2.94 and 2.00 μg/ml, respectively. Korean aldo keto reductase 1 b10 Ligularia fischeri var spiciformis extract oxidoreductase plant extract Rhus trichocarpa extract unclassified drug affinity chromatography article Asteraceae enzyme inhibition gel permeation chromatography Ligularia fischeri var spiciformis poison ivy Rhus trichocarpa Inhibitory effects of 23 Korean local plant extracts on aldo-keto reductase family 1 B10 (AKR 1 B10) Article 1 Korean Journal of Pharmacognosy, v.40, no.3, pp.238 - 243 Korean Journal of Pharmacognosy 40 3 238 243 scopus kci ART001382671 2-s2.0-76649139480 Article aldo keto reductase 1 b10 Ligularia fischeri var spiciformis extract oxidoreductase plant extract Rhus trichocarpa extract unclassified drug affinity chromatography article Asteraceae enzyme inhibition gel permeation chromatography Ligularia fischeri var spiciformis poison ivy Rhus trichocarpa Aldo-keto reductase family 1 B10 Aldose reductase-like 1 Anti-cancer Ligularia fischeri Rhus trichocarpa