Cha, Byung Hak Lee, Sang-Myung Park, Jae Chan Hwang, Kyo Seon Kim, Sang Kyung Lee, Yoon-Sik Ju, Byeong-Kwon Kim, Tae Song 2024-01-20T20:34:41Z 2024-01-20T20:34:41Z 2021-09-05 2009-09-15 0956-5663 https://pubs.kist.re.kr/handle/201004/132159 We report Hepatitis B Virus (HBV) DNA detection using a silica nanoparticle-enhanced dynamic micro-cantilever biosensor. A 243-mer nucleotide of HBV DNA precore/core region was used as the tar get DNA. For this assay, the capture probe on the microcantilever surface and the detection probe conjugated with silica nanoparticles were designed specifically for the target DNA. For efficient detection of the HBV target DNA using silica nanoparticle-enhanced DNA assay, the size of silica nanoparticles and the dimension of microcantilever were optimized by directly binding the silica nanoparticles through DNA hybridization. In addition, the correlation between the applied nanoparticle concentrations and the resonant frequency shifts of the microcantilever was discussed clearly to validate the quantitative relationship between mass loading and resonant frequency shift. HBV target DNAs of 23.1 fM to 2.31 nM which were obtained from the PCR product were detected using a silica nanoparticle-enhanced microcantilever. The HBV target DNA of 243-mer was detected up to the picomolar (pM) level without nanoparticle enhancement and up to the femtomolar (fM) level using a nanoparticle-based signal amplification process. In the above two cases, the resonant frequency shifts were found to be linearly correlated with the concentrations of HBV target DNAs. We believe that this linearity originated mainly from an increase in mass that resulted from binding between the probe DNA and HBV PCR product, and between HBV PCR product and silica nanoparticles for the signal enhancement, even though there is another potential factor such as the spring constant change that may have influenced on the resonant frequency of the microcantilever. (C) 2009 Published by Elsevier B.V. English ELSEVIER ADVANCED TECHNOLOGY LABEL-FREE NANOMECHANICAL MICROCANTILEVER CANTILEVER PROTEIN IMMUNOASSAY MICROARRAY PROBES GOLD Detection of Hepatitis B Virus (HBV) DNA at femtomolar concentrations using a silica nanoparticle-enhanced microcantilever sensor Article 10.1016/j.bios.2009.06.015 1 BIOSENSORS & BIOELECTRONICS, v.25, no.1, pp.130 - 135 BIOSENSORS & BIOELECTRONICS 25 1 130 135 scie scopus 000270538400021 2-s2.0-68049121836 Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry Science & Technology - Other Topics Article LABEL-FREE NANOMECHANICAL MICROCANTILEVER CANTILEVER PROTEIN IMMUNOASSAY MICROARRAY PROBES GOLD Dynamic microcantilever HBV DNA Sensitivity enhancement Sandwich assay Silica nanoparticles