Kim, Yong Ho Park, Chul-Kyu Back, Seung Keun Lee, C. Justin Hwang, Se Jin Bae, Yong Chul Na, Heung Sik Kim, Joong Soo Jung, Sung Jun Oh, Seog Bae 2024-01-20T21:01:43Z 2024-01-20T21:01:43Z 2021-09-03 2009-08-12 0270-6474 https://pubs.kist.re.kr/handle/201004/132242 Transient receptor potential vanilloid subtype 1 (TRPV1) and metabotropic glutamate receptor 5 (mGluR5) located on peripheral sensory terminals have been shown to play critical roles in the transduction and modulation of pain sensation. To date, however, very little is known regarding the significance of functional expression of mGluR5 and TRPV1 on the central terminals of sensory neurons in the dorsal horn of the spinal cord. Here we show that TRPV1 on central presynaptic terminals is coupled to mGluR5 in a membrane-delimited manner, thereby contributing to the modulation of nociceptive synaptic transmission in the substantia gelatinosa neurons of the spinal cord. Further, our results demonstrate that TRPV1 is involved in the pain behaviors induced by spinal mGluR5 activation, and diacylglycerol produced by the activation of mGluR5 mediates functional coupling of mGluR5 and TRPV1 on the presynaptic terminals. Thus, mGluR5-TRPV1 coupling on the central presynaptic terminals of nociceptive neurons may be an important mechanism underlying central sensitization under pathological pain conditions. English SOC NEUROSCIENCE METABOTROPIC GLUTAMATE RECEPTORS RAT SPINAL-CORD VANILLOID RECEPTOR DORSAL-HORN CAPSAICIN RECEPTOR SYNAPTIC-TRANSMISSION SUBSTANTIA-GELATINOSA SUPERFICIAL LAMINAE POTENTIAL CHANNELS DIRECT ACTIVATION Membrane-Delimited Coupling of TRPV1 and mGluR5 on Presynaptic Terminals of Nociceptive Neurons Article 10.1523/JNEUROSCI.5030-08.2009 1 JOURNAL OF NEUROSCIENCE, v.29, no.32, pp.10000 - 10009 JOURNAL OF NEUROSCIENCE 29 32 10000 10009 scie scopus 000268887300008 2-s2.0-68849094156 Neurosciences Neurosciences & Neurology Article METABOTROPIC GLUTAMATE RECEPTORS RAT SPINAL-CORD VANILLOID RECEPTOR DORSAL-HORN CAPSAICIN RECEPTOR SYNAPTIC-TRANSMISSION SUBSTANTIA-GELATINOSA SUPERFICIAL LAMINAE POTENTIAL CHANNELS DIRECT ACTIVATION TRPV1 mGluR5 Nociceptive Neuron presynaptic terminal