Saleem, Yasar Kim, Key-Sun 2024-01-20T21:02:23Z 2024-01-20T21:02:23Z 2021-09-03 2009-08 0006-291X https://pubs.kist.re.kr/handle/201004/132269 Regulator of G protein signaling 11 (RGS11) is the least characterized member of the R7 family of G gamma-like GGL domain-containing RGS proteins. All R7-RGS proteins of a variety of cell types are found in G beta 5-containing complexes that exhibit a number of unique functional properties. However, presence of G beta 5 reduced the affinity of R7-RGS7 for G alpha subunits, also only RGS7 bound to Muscarinic M3-Receptor, but the G beta 5-RGS7 dimer did not, making it difficult to study differential interaction of R7-RGS proteins. Here, we report the successful purification of functionally intact, G beta 5-free recombinant RGS11 (rRGS11), obtained by expressing N- and C-terminally truncated form of RGS11 in Escherichia coli BL 21 (DE3), that differentially interact with R7BP and G alpha(oa). rRGS11 was capable of interacting with G alpha(oa) and R7BP (RGS7 family binding protein) with equilibrium dissociation constants (K-D) of 904 (+/- 208) W, and 308 (+/- 97) nM, respectively. It also induced several-fold increase in the GTPase activity of G alpha(oa). The binding of rRGS11 was differential with a binding preference for R7BP over G alpha(oa) implying extended roles of R7BP. In addition, we identified a novel interaction between G(oa) and R7BP with a K-D of 592 (+/- 150) nM. The production of stable and functional rRGS11 would provide chances to discover more functions of RGS11 yet to be identified. (C) 2009 Published by Elsevier Inc. English ACADEMIC PRESS INC ELSEVIER SCIENCE RGS11 interacts preferentially with R7BP over G alpha(oa) - Characterization of G beta 5-free RGS11 Article 10.1016/j.bbrc.2009.05.128 1 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.386, no.1, pp.65 - 70 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 386 1 65 70 N scie scopus 000267868800014 2-s2.0-67649213475 Biochemistry & Molecular Biology Biophysics Biochemistry & Molecular Biology Biophysics Article MEMBRANE ANCHOR PROTEINS DOMAIN SELECTIVITY REGULATORS COMPLEXES RECEPTOR BINDING rRGS11 GAP activity R7BP SPR Differential interaction