Cho, Dae Haeng Lee, Yun Jie Um, Youngsoon Sang, Byoung-In Kim, Yong Hwan 2024-01-20T21:04:19Z 2024-01-20T21:04:19Z 2021-09-03 2009-07 0175-7598 https://pubs.kist.re.kr/handle/201004/132346 In the present study, we investigated the peroxidase-catalyzed detoxification of model phenolic compounds and evaluated the inhibitory effects of the detoxified solution on butanol production by Clostridium beijerinckii National Collection of Industrial and Marine Bacteria Ltd. 8052. The six phenolic compounds, p-coumaric acid, ferulic acid, 4-hydroxybenzoic acid, vanillic acid, syringaldehyde, and vanillin, were selected as model fermentation inhibitors generated during pretreatment and hydrolysis of lignocellulose. The enzyme reaction was optimized as a function of the reaction conditions of pH, peroxidase concentration, and hydrogen peroxide to substrate ratio. Most of the tested phenolics have a broad optimum pH range of 6.0 to 9. Removal efficiency increased with the molar ratio of H2O2 to each compound up to 0.5-1.25. In the case of p-coumaric acid, ferulic acid, vanillic acid, and vanillin, the removal efficiency was almost 100% with only 0.01 mu M of enzyme. The tested phenolic compounds (1 g/L) inhibited cell growth by 64-74%, while completely inhibiting the production of butanol. Although syringaldehyde and vanillin were less toxic on cell growth, the level of inhibition on the butanol production was quite different. The detoxified solution remarkably improved cell growth and surprisingly increased butanol production to the level of the control. Hence, our present study, using peroxidase for the removal of model phenolic compounds, could be applied towards the detoxification of lignocellulosic hydrolysates for butanol fermentation. English SPRINGER Detoxification of model phenolic compounds in lignocellulosic hydrolysates with peroxidase for butanol production from Clostridium beijerinckii Article 10.1007/s00253-009-1925-8 1 APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.83, no.6, pp.1035 - 1043 APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 83 6 1035 1043 N scie scopus 000267679900006 2-s2.0-67650714001 Biotechnology & Applied Microbiology Biotechnology & Applied Microbiology Article COPRINUS-CINEREUS PEROXIDASE ION-EXCHANGE-RESINS XYLITOL PRODUCTION CATALYZED POLYMERIZATION DEGRADATION-PRODUCTS CELLULOSE HYDROLYSIS ESCHERICHIA-COLI FERMENTATION GROWTH INHIBITION Lignocellulosic hydrolysates Phenolic compound Peroxidase Butanol Clostridium beijerinckii Inhibitor