Baek, Je-Hyun Kim, Hokeun Shin, Byunghee Yu, Myeong-Hee 2024-01-20T21:04:50Z 2024-01-20T21:04:50Z 2021-09-03 2009-07 1535-3893 https://pubs.kist.re.kr/handle/201004/132368 Quantification of target peptides and proteins is crucial for biomarker discovery. Approaches such as selected reaction monitoring (SRM) and multiple reaction monitoring (MRM) rely on liquid chromatography and mass spectrometric analysis of defined peptide product ions. These methods are not very widespread because the determination of quantifiable product ion using either SRM or MRM is a very time-consuming process. We developed a novel approach for quantifying target peptides without such an arduous process of ion selection. This method is based on monitoring multiple product ions (multiple products monitoring: MpM) from full-range MS2 spectra of a target precursor. The MpM method uses a scoring system that considers both the absolute intensities of product ions and the similarities between the query MS2 spectrum and the reference MS2 spectrum of the target peptide. Compared with conventional approaches, MpM greatly improves sensitivity and selectivity of peptide quantification using an ion-trap mass spectrometer. English AMER CHEMICAL SOC SPECTROMETRY-BASED PROTEOMICS QUANTITATIVE-ANALYSIS MASS-SPECTROMETRY ABSOLUTE QUANTIFICATION ION-TRAP PROTEINS MIXTURES STRATEGY SPECTRA MS/MS Multiple Products Monitoring as a Robust Approach for Peptide Quantification Article 10.1021/pr800853k 1 JOURNAL OF PROTEOME RESEARCH, v.8, no.7, pp.3625 - 3632 JOURNAL OF PROTEOME RESEARCH 8 7 3625 3632 scie scopus 000267694600037 2-s2.0-67650361220 Biochemical Research Methods Biochemistry & Molecular Biology Article SPECTROMETRY-BASED PROTEOMICS QUANTITATIVE-ANALYSIS MASS-SPECTROMETRY ABSOLUTE QUANTIFICATION ION-TRAP PROTEINS MIXTURES STRATEGY SPECTRA MS/MS peptide quantification product ion monitoring multiple products monitoring, target verification