Kang, Gum-Yong Lee, Eung-Ryoung Kim, Jung-Hyun Jung, Jin Woo Lim, Jun Kim, Sang Kyung Cho, Ssang-Goo Kim, Kwang Pyo 2024-01-20T21:05:40Z 2024-01-20T21:05:40Z 2021-09-03 2009-06-02 0014-2999 https://pubs.kist.re.kr/handle/201004/132402 The molecular mechanisms underlying the kaempferol-induced cell death have not yet been fully explained. To investigate the role of kaempferol, widely distributed in foods, in tumor progression, human breast cancer cell line, MCF-7, was treated with kaempferol. Apoptosis was indicated by the accumulation of a sub-G1 population, as well as the appearance of 4'-6-diamidino-2-phenylindole (DAPI)-stained apoptotic nuclei in the MCF-7 cells after the administration of kaempferol. Western blot analysis showed cleavage of Poly (ADPribose) polymerase (PARP), caspase-7, Bax. and caspase-9 indicating that the intracellular pathway of apoptosis was involved. Kaempferol also downregulated the expression of polo-like kinase 1 (PLK-1), which has been reported to regulate mitotic progression and to be upregulated in several human tumors. Taken together, these findings indicate that kaempferol-induced apoptosis by initiation of intrinsic caspase cascade and downregulation of PLK-1 expression. (C) 2009 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV POLO-LIKE-KINASE CANCER-CELLS PROGNOSTIC-SIGNIFICANCE GROWTH-INHIBITION PROSTATE-CANCER PROTEIN-KINASE LUNG-CANCER FLAVONOIDS PROLIFERATION KERATINOCYTES Downregulation of PLK-1 expression in kaempferol-induced apoptosis of MCF-7 cells Article 10.1016/j.ejphar.2009.03.068 1 EUROPEAN JOURNAL OF PHARMACOLOGY, v.611, no.1-3, pp.17 - 21 EUROPEAN JOURNAL OF PHARMACOLOGY 611 1-3 17 21 scie scopus 000266539600003 2-s2.0-67349252446 Pharmacology & Pharmacy Pharmacology & Pharmacy Article POLO-LIKE-KINASE CANCER-CELLS PROGNOSTIC-SIGNIFICANCE GROWTH-INHIBITION PROSTATE-CANCER PROTEIN-KINASE LUNG-CANCER FLAVONOIDS PROLIFERATION KERATINOCYTES Apoptosis Kaempferol Polo-like kinase 1 Caspase Poly-ADP-ribose polymerase