Yi, Jae Kyo Chang, Jong Wook Han, Wonshik Lee, Jong Won Ko, Eunyoung Kim, Dong Hyun Bae, Ji-Yeon Yu, Jonghan Lee, Cheolju Yu, Myeong-Hee Noh, Dong-Young 2024-01-20T21:32:15Z 2024-01-20T21:32:15Z 2021-09-03 2009-05 1055-9965 https://pubs.kist.re.kr/handle/201004/132528 We sought to identify a new serum biomarker for breast cancer screening and diagnosis using stepwise proteomic analysis of sera from breast cancer patients to detect the presence of autoantibodies that react with urinary protein. Two-dimensional immunoblotting was done for screening autoimmunogenic tumor antigens in the urine of breast cancer patients. Reactive spots were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Among urinary proteins separated by two-dimensional electrophoresis, 13 spots showed strong reactivity with pooled sera from breast cancer patients or control sera. By mass spectrometry, we identified alpha 2-HS glycoprotein (AHSG) as a tumor antigen. Peripheral blood was obtained from 81 women diagnosed with breast cancer before surgery and 73 female donors without evidence of any malignancy for the individual analysis. In one-dimensional Western blot analysis, AHSG autoantibody was detected in 64 of 81 breast cancer patients (79.1%) and in 7 of 73 controls (9.6%). The sensitivity of this test in breast cancer patients was 79.0%. Our results suggest that AHSG and anti-AHSG autoantibody may be useful serum biomarkers for breast cancer screening and diagnosis. (Cancer Epidemiol Biomarkers Prev 2009;18(5):1357-64) English AMER ASSOC CANCER RESEARCH PROTEOMICS-BASED APPROACH IMMUNE-RESPONSE 2-DIMENSIONAL ELECTROPHORESIS INSULIN-RECEPTOR LUNG-CANCER CATHEPSIN-D MARKERS PROTEIN IDENTIFICATION EXPRESSION Autoantibody to Tumor Antigen, Alpha 2-HS Glycoprotein: A Novel Biomarker of Breast Cancer Screening and Diagnosis Article 10.1158/1055-9965.EPI-08-0696 1 CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION, v.18, no.5, pp.1357 - 1364 CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION 18 5 1357 1364 scie scopus 000266081500004 2-s2.0-66549089699 Oncology Public, Environmental & Occupational Health Oncology Public, Environmental & Occupational Health Article PROTEOMICS-BASED APPROACH IMMUNE-RESPONSE 2-DIMENSIONAL ELECTROPHORESIS INSULIN-RECEPTOR LUNG-CANCER CATHEPSIN-D MARKERS PROTEIN IDENTIFICATION EXPRESSION