Krishnan, Jegatha Nambi Kim, Choong Park, Hyun Jik Kang, Ji Yoon Kim, Tae Song Kim, Sang Kyung 2024-01-20T21:32:23Z 2024-01-20T21:32:23Z 2021-09-03 2009-05 0173-0835 https://pubs.kist.re.kr/handle/201004/132534 We present the design and fabrication of a new microfluidic device in which the dielectrophoresis and magnetophoresis phenomena were used for the separation of the superparamagnetic microbeads of different sizes. By exploiting the fact that two different particles can exhibit different dielectrophoretic force-frequency spectra, we utilize this device to perform multiplex detection from a single sample solution. We found the transition frequency range for 1, 2.8, and 4.5 mu m magnetic beads using our device. Bead-based analysis revealed that a high separation efficiency (similar to 90%) could be obtained from a single sample solution containing both 4.5 and 2.8 mu m beads. The average flow velocity of the beads was maintained at 9.8 mm/s, enabling fast analysis with a smaller amount of reagents. The magnetic field distribution on the beads and the bead flow at the channel cross section for different dielectrophoretic conditions was obtained using CFD-ACE(+) simulation. Issues relating to the fabrication and operation of the device are discussed in detail. Finally, we demonstrated the feasibility of parallel detection/trapping of different beads on the same chip. This separation approach offers the performance of multiplex analysis in lab-on-a-chip devices. English WILEY ON-CHIP Rapid microfluidic separation of magnetic beads through dielectrophoresis and magnetophoresis Article 10.1002/elps.200800646 1 ELECTROPHORESIS, v.30, no.9, pp.1457 - 1463 ELECTROPHORESIS 30 9 1457 1463 scie scopus 000266312800004 2-s2.0-69249216812 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article ON-CHIP Dielectrophoresis Magnetophoresis Microfluidics Superparamagnetic microbeads