Kim, Choong Lee, Kang Sun Kim, Young Eun Lee, Kyu-Jung Lee, Soo Hyun Kim, Tae Song Kang, Ji Yoon 2024-01-20T21:32:32Z 2024-01-20T21:32:32Z 2021-09-03 2009-05 1473-0197 https://pubs.kist.re.kr/handle/201004/132540 This paper describes a microfluidic device for the microencapsulation of cells in alginate beads to enhance cell viability. The alginate droplet including cells was gelified with calcified oleic acid, using two-phase microfluidics. The on-chip gelation had generated monodisperse spherical alginate beads, which could not be readily obtained via conventional external gelation in a calcium chloride bath. However, the prolonged exposure of encapsulated cells to the toxic oil phase caused serious damage to the cells. Therefore, we proposed the formulation of a rapid oil-exchange chip which transforms the toxic oleic acid to harmless mineral oil. The flushing out of oleic acid after the gelation of alginate beads effected a dramatic increase in the viability of P19 embryonic carcinoma cells, up to 90%. The experimental results demonstrated that the cell viability was proportional to the flow rate of squeezing mineral oil. English Royal Society of Chemistry MICROFLUIDIC DEVICE ENCAPSULATION MICROBEADS DIFFERENTIATION MICROSPHERES Rapid exchange of oil-phase in microencapsulation chip to enhance cell viability Article 10.1039/b819044e 1 Lab on a Chip, v.9, no.9, pp.1294 - 1297 Lab on a Chip 9 9 1294 1297 scie scopus 000265223200022 2-s2.0-65349196129 Biochemical Research Methods Chemistry, Multidisciplinary Chemistry, Analytical Nanoscience & Nanotechnology Instruments & Instrumentation Biochemistry & Molecular Biology Chemistry Science & Technology - Other Topics Instruments & Instrumentation Article MICROFLUIDIC DEVICE ENCAPSULATION MICROBEADS DIFFERENTIATION MICROSPHERES