Nam, Ki Hyun Kim, Kook-Han Kim, Eunice Eun Kyeong Hwang, Kwang Yeon 2024-01-20T21:33:10Z 2024-01-20T21:33:10Z 2021-09-03 2009-04-17 0006-291X https://pubs.kist.re.kr/handle/201004/132563 PDF (peptide deformylase) plays a critical role in the production of mature proteins by removing the N-formyl polypeptide of nascent proteins in the prokaryote cell system. This protein is essential for bacterial growth, making it an attractive target for the design of new antibiotics. Accordingly, PDF has been evaluated as a drug target; however, architectural mechanism studies of PDF have not yet fully elucidated its molecular function. We recently reported the crystal structure of PDF produced by Enterococcus faecium [K.H. Nam, J.I. Ham, A. Priyadarshi, E.E. Kim, N. Chung, K.Y. Hwang, "Insight into the antibacterial drug design and architectural mechanism of peptide recognition from the E. faecium peptide deformylase structure", Proteins 74 (2009) 261-265]. Here, we present the crystal structure of the EfPDF complex with MAS (Met-Ser-Ala), thereby not only delineating the architectural mechanism for the recognition of mimic-peptides by N-terminal cleaved expression peptide, but also suggesting possible targets for rational design of antibacterial drugs. In addition to their implications for drug design, these structural studies will facilitate elucidation of the architectural mechanism responsible for the peptide recognition of PDF. (C) 2009 Elsevier Inc. All rights reserved. English ACADEMIC PRESS INC ELSEVIER SCIENCE PEPTIDE DEFORMYLASE INHIBITORS STAPHYLOCOCCUS-AUREUS ANTIBACTERIAL AGENTS ACTIVE-SITE DRUG DESIGN IN-VITRO IDENTIFICATION ACTINONIN FORMYLTRANSFERASE ANTIBIOTICS Crystal structure of an EfPDF complex with Met-Ala-Ser based on crystallographic packing Article 10.1016/j.bbrc.2009.02.113 1 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.381, no.4, pp.630 - 633 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 381 4 630 633 scie scopus 000264929400034 2-s2.0-62649139034 Biochemistry & Molecular Biology Biophysics Biochemistry & Molecular Biology Biophysics Article PEPTIDE DEFORMYLASE INHIBITORS STAPHYLOCOCCUS-AUREUS ANTIBACTERIAL AGENTS ACTIVE-SITE DRUG DESIGN IN-VITRO IDENTIFICATION ACTINONIN FORMYLTRANSFERASE ANTIBIOTICS PDF Peptide deformylase EfPDF-MAS Antibacterial drug design