Nugraha, Boya Yoon, Aerin Kandagaddala, Lakshmi Devi Cho, Hyo Joo Chung, Bong Chul Kwon, Oh-Seung 2024-01-20T21:34:15Z 2024-01-20T21:34:15Z 2021-09-03 2009-04 1976-9148 https://pubs.kist.re.kr/handle/201004/132608 This work was conducted to investigate the effect of bisphenol A (BPA) on estradiol (E2) 2-and 4-hydroxylase activities in the liver, kidney and lung tissues of male and female rats. After intraperitoneal administration of BPA to male and female rats for 4 days at 0, 10, and 50 mg/kg, the conversion of the substrate for hepatic and extra-hepatic enzyme activities was measured by GC/MSD. The result showed decreases of body and organ weights at 50 mg/kg BPA of male and female rats. Male hepatic E2 2-hydroxylase activity was inhibited by 68% at 10 mg/kg and by 82% at 50 mg/kg BPA. Female hepatic E2 2-hydroxylase activity was decreased by 46% at 10 mg/kg and by 56% at 50 mg/kg to the control. E2 4-hydroxylase was inhibited by 57 and 57% at 10 mg/kg and 54 and 78% at 50 mg/kg in liver of female and male, respectively. The urinary excretion rate of 2-hydroxyestradiol (2-OHE), androsterone and testosterone in urine of female rats with 50 mg/kg BPA were decreased significantly. The results showed that 50 mg/kg BPA was decreased E2 2-and 4-hydroxylase activities in liver, but not in other tissues. The urinary excretion rates of 2-OHE, androsterone and testosterone were also decreased. In liver, estrogenic enzyme activity were higher in male than female. These results suggest that BPA can disrupt estrogen metabolism by suppressing E2 2-and 4-hydroxylase activities in the liver of male and female rats. English KOREAN SOC APPLIED PHARMACOLOGY In Vivo Suppression of Bisphenol A on Estradiol 2-and 4-Hydroxylase Activities in Hepatic Microsomal Fractions of Male and Female Sprague-Dawley Rats Article 10.4062/biomolther.2009.17.2.188 1 BIOMOLECULES & THERAPEUTICS, v.17, no.2, pp.188 - 198 BIOMOLECULES & THERAPEUTICS 17 2 188 198 N scie scopus kci ART001338751 000266067200012 2-s2.0-69549116182 Biochemistry & Molecular Biology Pharmacology & Pharmacy Biochemistry & Molecular Biology Pharmacology & Pharmacy Article ESTROGEN-RECEPTOR-ALPHA LIVER-MICROSOMES CYTOCHROME-P450 ISOFORMS METABOLISM CELLS 17-BETA-ESTRADIOL VITRO MICE 2-METHOXYESTRADIOL ANGIOGENESIS Bisphenol A Estradiol 2-hydroxylase Estradiol 4-hydroxylase Estrogen metabolites Rat