Kim, Su-Nam Choi, Hye-Young Kim, Yong Kee 2024-01-20T21:34:23Z 2024-01-20T21:34:23Z 2021-09-03 2009-04 0253-6269 https://pubs.kist.re.kr/handle/201004/132613 In this study, we investigated the effects of various histone deacetylase (HDAC) inhibitors on adipocyte differentiation. Treatment of 3T3-L1 cells with HDAC inhibitors such as apicidin, trichostatin A, or suberoylanilide hydroxamic acid, under conditions that normally promote differentiation led to a dramatic attenuation of adipocyte differentiation. In contrast, sodium butyrate (NaB) treatment increased adipocyte differentiation. Accordingly, the expression of adipogenic marker genes such as FAS, aP2, PPAR gamma, resistin, C/EBP alpha, ADD1/SREBP1c, and adiponectin were inhibited by apicidin treatment but not NaB, indicating that the adipocyte differentiation process could be differentially regulated depending on the type of HDAC inhibitor utilized. In addition, this differential effect seemed not to be due to disruption of the insulin- signaling pathway. Interestingly, our data showed that apicidin treatment could induce dedifferentiation of fully differentiated adipocytes, as evident by the fact that apicidin treatment led to a decrease of Oil Red O-stained adipocytes with a concomitant reduction in the expression levels of adipogenic marker genes. Collectively, our results suggest that adipocyte differentiation and dedifferentiation may be regulated by HDAC inhibitors. English PHARMACEUTICAL SOC KOREA SP1 SITES TRANSCRIPTIONAL REGULATION EXPRESSION APICIDIN RECEPTOR PROTEIN KINASE ADIPOGENESIS ACTIVATION PATHWAYS Regulation of adipocyte differentiation by histone deacetylase inhibitors Article 10.1007/s12272-009-1409-5 1 ARCHIVES OF PHARMACAL RESEARCH, v.32, no.4, pp.535 - 541 ARCHIVES OF PHARMACAL RESEARCH 32 4 535 541 scie scopus kci ART001336201 000265683200011 2-s2.0-65549117436 Chemistry, Medicinal Pharmacology & Pharmacy Pharmacology & Pharmacy Article SP1 SITES TRANSCRIPTIONAL REGULATION EXPRESSION APICIDIN RECEPTOR PROTEIN KINASE ADIPOGENESIS ACTIVATION PATHWAYS Adipocyte differentiation Dedifferentiation HDAC inhibitors Apicidin NaB