Cho, Sung-Hee Choi, Man Ho Lee, Won-Yong Chung, Bong Chul 2024-01-20T21:34:38Z 2024-01-20T21:34:38Z 2021-09-03 2009-04 0009-9120 https://pubs.kist.re.kr/handle/201004/132624 Objective: Evaluation of altered urinary nucleosides before and after tumor removal of breast cancer (BCa). Design and methods: Targeted metabolite profiling of 14 urinary nucleosides was conducted with both pre- and post-operative female patients with BCa (n = 150, age: 46.6 +/- 7.7 years), and female controls (n = 150, age: 46.8 +/- 7.7 years) by liquid chromatography-tandem mass spectrometry coupled to on-line extraction. Results: Levels of modified nucleosides (5-hydroxymethyl-2&apos;-deoxyuridine, P<0.001 8-hydroxy-2&apos;-deoxyguanosine, P<0.001; 1-methyladenosine, P<0.02; N-2,N-2-dimethylguanosine, P<0.001) were significantly higher in pre-operative patients than in both normal controls and post-operative patients. Conclusions: This approach could be used to further understand the pathogenesis of BCa as well as to evaluate the effects of medical treatment. (C) 2009 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved. English PERGAMON-ELSEVIER SCIENCE LTD DNA SPECTROMETRY MARKER Evaluation of urinary nucleosides in breast cancer patients before and after tumor removal Article 10.1016/j.clinbiochem.2008.12.026 1 CLINICAL BIOCHEMISTRY, v.42, no.6, pp.540 - 543 CLINICAL BIOCHEMISTRY 42 6 540 543 scie scopus 000264538300016 2-s2.0-61849178717 Medical Laboratory Technology Medical Laboratory Technology Article DNA SPECTROMETRY MARKER Nucleosides Breast cancer DNA damage Liquid chromatography-mass spectrometry