Kim, Yun-Kyoung Oh, Soo-Jin Jin, Bong-Suk Park, Chanhoo Jeon, Hyesung Boo, Doo Wan Yu, Yeon Gyu 2024-01-20T21:35:07Z 2024-01-20T21:35:07Z 2021-09-03 2009-03-20 0253-2964 https://pubs.kist.re.kr/handle/201004/132643 Interactions between the surface and capsid proteins of the hepatitis B virus (HBV) are critical for the assembly of virus particles. In this study, we developed a cell-based method to visualize the interactions between the capsid and surface proteins of HBV. Capsid-GFP, a capsid protein fused to a green fluorescence protein (GFP), forms nucleocapsid-like structures in the cytoplasm of mammalian cells. It relocates to the plasma membranes in cells expressing PH-PreS, a fusion protein consisting of the PreS region of the HBV surface protein and the PH domain of PLC-gamma. Membrane localization of the capsid-GFP in these cells is prevented by an inhibitory peptide that blocks the interaction between the capsid and surface proteins. This dynamic localization of capsid-GFP is applicable for screening compounds that may potentially inhibit or prevent the assembly process of HBV particles. English WILEY-V C H VERLAG GMBH DOMINANT-NEGATIVE MUTANTS PRE-S DOMAIN CORE PROTEIN PLASMA-MEMBRANE ANTIGEN ASSOCIATION PARTICLES SEQUENCE A Cell-based Method to Monitor the Interaction between Hepatitis B Virus Capsid and Surface Proteins Article 1 BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.30, no.3, pp.577 - 581 BULLETIN OF THE KOREAN CHEMICAL SOCIETY 30 3 577 581 scie scopus kci ART001524224 000265240000011 2-s2.0-73249141381 Chemistry, Multidisciplinary Chemistry Article DOMINANT-NEGATIVE MUTANTS PRE-S DOMAIN CORE PROTEIN PLASMA-MEMBRANE ANTIGEN ASSOCIATION PARTICLES SEQUENCE HBV PreS Capsid Interaction