Hong, Jongin Yoon, Daesung Kim, Tae Song 2024-01-20T21:35:14Z 2024-01-20T21:35:14Z 2021-09-01 2009-03-20 1976-0280 https://pubs.kist.re.kr/handle/201004/132647 The C-reactive protein (CRP) is a very important inflammation biomarker and a major predictor of cardiovascular disease (CVD) in human. We demonstrated here on a new approach to determine the binding of label free CRP to monoclonal anti-CRP using the Mach-Zehnder interferometer based on silicon oxides with the detection limit of 1 ng/mL or even less at the operating wavelength of 1,550 nm. The building block, single-mode waveguide of total internal reflection, was precisely designed using a finite element method (FEM) and so we suggested the guideline of micro-fabrication to achieve it. We also firstly introduced the calixcrown self-assembled monolayers (SAMs), which guaranteed the activity of capture proteins and the correct orientation, to immobilize the monoclonal anti-CRP on PECVD silicon oxides into the biosensors based on the interaction of the evanescent field of the guided light with the surrounding environment. English KOREAN BIOCHIP SOCIETY-KBCS ANTIBODY-ANTIGEN INTERACTION ELECTRICAL DETECTION IMMUNOSENSORS SENSITIVITY DISEASE MARKER The Mach-Zehnder Interferometer Based on Silicon Oxides for Label Free Detection of C-reactive Protein (CRP) Article 1 BIOCHIP JOURNAL, v.3, no.1, pp.1 - 11 BIOCHIP JOURNAL 3 1 1 11 scie scopus kci other 000264435600001 2-s2.0-77952551993 Biochemical Research Methods Chemistry, Analytical Nanoscience & Nanotechnology Biochemistry & Molecular Biology Chemistry Science & Technology - Other Topics Article ANTIBODY-ANTIGEN INTERACTION ELECTRICAL DETECTION IMMUNOSENSORS SENSITIVITY DISEASE MARKER C-reactive protein Mach-Zehnder interferometer Biosensor Finite element method Silicon oxide