Islam, M. Nurul Yoo, Hye Hyun Sung, Chung-Ki Dong, Mi-sook Park, Young In Jin, Changbae Kim, Dong-Hyun 2024-01-20T21:36:00Z 2024-01-20T21:36:00Z 2021-09-03 2009-03 1060-3271 https://pubs.kist.re.kr/handle/201004/132679 This paper describes a simple, rapid, and validated reversed-phase high-performance liquid chromatographic method developed for the determination of 4 major bioactive constituents, namely, chlorogenic acid, ferulic acid, senkyunolide A, and Z-ligustilide in Rhizoma Cnidii extract. A Capcell Pak C-18 chromatographic column (150 x 4.6 mm, 3 mu m) was used with mobile phases consisting of 0.1% formic acid, acetonitrile, and methanol at a flow rate of 0.8 mL/min and UV detection at 285 nm. Comprehensive validation of the method included evaluation of linearity, repeatability, recovery, and stability. Excellent linear behavior (r(2) >0.99) was observed over the concentration range of 2-100 mu g/mL for the compounds under investigation. Repeatability and accuracy were evaluated by intra- and interday assays; the relative standard deviation (RSD) values were <= 5.37% and accuracies ranged from 97.1 to 104.9%. Recoveries of the compounds ranged from 94.2 to 104.2% with RSD values of <= 9.50%. The developed method was successfully applied to the analysis of ethanolic extracts of Rhizoma Cnidii samples. As a result, the concentrations of chlorogenic acid, ferulic acid, Z-ligustilide, and senkyunolide A were determined to be 0.84-5.35, 0.45-1.65, 0.74-4.39, and 0.32-1.14 mg/g herb, respectively. Thus, the developed method was found to be accurate and reproducible and is considered suitable for the qualitative and quantitative analysis of Rhizoma Cnidii for bioactive compounds. English AOAC INT UMBELLIFEROUS MEDICINAL-PLANTS LIGUSTICUM-CHUANXIONG OFFICINALE MAKINO ANGELICA QUANTIFICATION SENKYUNOLIDE CONSTITUENTS FINGERPRINTS COMPONENTS CULTURES Simultaneous Determination of Phenolic Acids and Phthalide Compounds by Liquid Chromatography for Quality Assessment of Rhizoma Cnidii Article 1 JOURNAL OF AOAC INTERNATIONAL, v.92, no.2, pp.375 - 381 JOURNAL OF AOAC INTERNATIONAL 92 2 375 381 scie scopus 000265309100003 2-s2.0-69249174331 Chemistry, Analytical Food Science & Technology Chemistry Food Science & Technology Article UMBELLIFEROUS MEDICINAL-PLANTS LIGUSTICUM-CHUANXIONG OFFICINALE MAKINO ANGELICA QUANTIFICATION SENKYUNOLIDE CONSTITUENTS FINGERPRINTS COMPONENTS CULTURES