Cho, Sung-Hee
Choi, Man Ho
Kwon, Oh Seung
Lee, Won-Yong
Chung, Bong Chul
2024-01-20T21:36:09Z
2024-01-20T21:36:09Z
2021-09-03
2009-03
0260-437X
https://pubs.kist.re.kr/handle/201004/132685
Modified nucleosides are formed by DNA repair as a result of oxidative DNA damage and post-transcriptionally modified tRNA in cells. In the present study, profiling of 14 nucleoside was investigated in rat urine to evaluate bisphenol A (BPA)-induced oxidative stress after intraperitoneally injecting rats with 0, 10 or 50 mg kg(-1) per day of BPA for four consecutive days. The urinary concentrations of individual nucleosides were measured by liquid chromatography-tandem mass spectrometry combined with column switching on-line extraction. Increased levels of 5-hydroxymethyi-2'-deoxyuridine (P < 0.01 on first, P < 0.005 on second, P < 0.001 on third and P < 0.01 on fourth day) and 8-hydroxy-2'-deoxyguanosine (P < 0.005 on second, P < 0.001 on third and P < 0.001 on fourth day) were found. Also, the patterns of urinary nucleosides in three dosage groups (control, BPA1, BPA2) were significantly different. Statistical significance was observed between BPA1 (5-hydroxymethyl-2'-deoxyuridine, P < 0.05; 8-hydroxy-2'-deoxyguanosine, P < 0.005) and BPA2 (5-hydroxymethyl-2'-deoxyurindine, P < 0.005; 8-hydroxy-2'-deoxyguanosine, P < 0.001) during the treatment period. Supervised analysis with partial least-squares-discrimination analysis led to discrimination between the three dosage groups. Quantitative alterations showed the metabolic trajectories responsible for physiological responses. The described methods could be used to evaluate and monitor BPA-induced oxidative stress early after exposure. Copyright (C) 2008 John Wiley & Sons, Ltd.
English
WILEY
Metabolic significance of bisphenol A-induced oxidative stress in rat urine measured by liquid chromatography-mass spectrometry
Article
10.1002/jat.1387
1
JOURNAL OF APPLIED TOXICOLOGY, v.29, no.2, pp.110 - 117
JOURNAL OF APPLIED TOXICOLOGY
29
2
110
117
N
scie
scopus
000263803400003
2-s2.0-62849108018
Toxicology
Toxicology
Article
ESTROGEN-LIKE CHEMICALS
DNA-DAMAGE
FREE-RADICALS
GUANINE BASES
LIVER
METABONOMICS
8-HYDROXYLATION
GENERATION
TOXICITY
EXPOSURE
bisphenol A
nucleosides
oxidative stress
trajectory analysis
liquid chromatography
mass spectrometry