Jung, Hyun-Jin Lee, Won-Yong Chung, Bong Chul Choi, Man Ho 2024-01-20T22:01:04Z 2024-01-20T22:01:04Z 2021-09-03 2009-02-27 0021-9673 https://pubs.kist.re.kr/handle/201004/132727 An efficient analytical method for simultaneous determination of 12 SFEs in serum is described. The method involves solid-phase extraction to isolate of SFEs from interfering species, especially cholesteryl esters, conversion to trimethylsilyl (TMS) ether derivatives for the direct analysis by gas chromatography-mass spectrometry (GC-MS) using a high temperature MXT-1 (Silcosteel-treated stainless steel) capillary column. All SFEs as their TMS derivatives were well separated with excellent peak shapes within 12 min. Overall recoveries ranged from 88% to 119%, with a detection limits for SFEs ranged from 2 to 30 mu g L-1. The linearity as correlation coefficient was higher than 0.99 except for pregnenolone-3-arachidate (r(2) = -0.98) in the concentration range of 5-3000 mu g L-1. Ten serum samples obtained from volunteers were also analyzed and quantitatively determined of DHEA-3-palmitate and pregnenolone-3-stearate in 1.8-1195.8 mu g L-1 concentration. The devised high temperature GC-MS method could be useful for identification of SFEs in biological specimens including serum. (C) 2008 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV LIQUID-CHROMATOGRAPHY SAMPLE PREPARATION FOLLICULAR-FLUID PREGNENOLONE IDENTIFICATION ESTRADIOL RAT ESTERIFICATION NEUROSTEROIDS LIPOPROTEINS Mass spectrometric profiling of saturated fatty acid esters of steroids separated by high-temperature gas chromatography Article 10.1016/j.chroma.2008.12.059 1 JOURNAL OF CHROMATOGRAPHY A, v.1216, no.9, pp.1463 - 1468 JOURNAL OF CHROMATOGRAPHY A 1216 9 1463 1468 scie scopus 000263610500025 2-s2.0-59049106573 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article LIQUID-CHROMATOGRAPHY SAMPLE PREPARATION FOLLICULAR-FLUID PREGNENOLONE IDENTIFICATION ESTRADIOL RAT ESTERIFICATION NEUROSTEROIDS LIPOPROTEINS Steroids Fatty acids Lipoidal conjugate Gas chromatography Mass spectrometry