Chiang, Chien-Sung Huang, Ching-Hui Chieng, Hockling Chang, Ya-Ting Chang, Dory Chen, Ji-Jr Chen, Yong-Cyuan Chen, Yen-Hui Shin, Hee-Sup Campbell, Kevin P. Chen, Chien-Chang 2024-01-20T22:01:05Z 2024-01-20T22:01:05Z 2021-09-03 2009-02-27 0009-7330 https://pubs.kist.re.kr/handle/201004/132728 Voltage-gated T-type Ca2+ channels (T-channels) are normally expressed during embryonic development in ventricular myocytes but are undetectable in adult ventricular myocytes. Interestingly, T-channels are reexpressed in hypertrophied or failing hearts. It is unclear whether T-channels play a role in the pathogenesis of cardiomyopathy and what the mechanism might be. Here we show that the alpha(1H) voltage-gated T-type Ca2+ channel (Ca(v)3.2) is involved in the pathogenesis of cardiac hypertrophy via the activation of calcineurin/nuclear factor of activated T cells (NFAT) pathway. Specifically, pressure overload-induced hypertrophy was severely suppressed in mice deficient for Ca(v)3.2 (Ca(v)3.2(-/-)) but not in mice deficient for Ca(v)3.1 (Ca(v)3.1(-/-). Angiotensin II-induced cardiac hypertrophy was also suppressed in Ca(v)3.2(-/-) mice. Consistent with these findings, cultured neonatal myocytes isolated from Ca(v)3.2(-/-) mice fail to respond hypertrophic stimulation by treatment with angiotensin II. Together, these results demonstrate the importance of Ca(v)3.2 in the development of cardiac hypertrophy both in vitro and in vivo. To test whether Ca(v)3.2 mediates the hypertrophic response through the calcineurin/NFAT pathway, we generated Ca(v)3.2(-/-), NFAT-luciferase reporter mice and showed that NFAT-luciferase reporter activity failed to increase after pressure overload in the Ca(v)3.2(-/-)/NFAT-Luc mice. Our results provide strong genetic evidence that Ca(v)3.2 indeed plays a pivotal role in the induction of calcineurin/NFAT hypertrophic signaling and is crucial for the activation of pathological cardiac hypertrophy. (Circ Res. 2009;104:522-530.) English LIPPINCOTT WILLIAMS & WILKINS CALCIUM-CHANNEL CARDIOMYOPATHIC HAMSTER MYOCARDIAL-INFARCTION INTRACELLULAR CALCIUM VENTRICULAR MYOCYTES MOUSE HEART IN-VIVO CALCINEURIN EXPRESSION ACTIVATION The Ca(v)3.2 T-Type Ca2+ Channel Is Required for Pressure Overload-Induced Cardiac Hypertrophy in Mice Article 10.1161/CIRCRESAHA.108.184051 1 CIRCULATION RESEARCH, v.104, no.4, pp.522 - U194 CIRCULATION RESEARCH 104 4 522 U194 scie scopus 000263771600015 2-s2.0-61949111681 Cardiac & Cardiovascular Systems Hematology Peripheral Vascular Disease Cardiovascular System & Cardiology Hematology Article CALCIUM-CHANNEL CARDIOMYOPATHIC HAMSTER MYOCARDIAL-INFARCTION INTRACELLULAR CALCIUM VENTRICULAR MYOCYTES MOUSE HEART IN-VIVO CALCINEURIN EXPRESSION ACTIVATION hypertrophy cardiomyopathy T-type Ca2+ channel