Park, Hyun Bong Kwon, Hak Cheol Lee, Chang-Ho Yang, Hyun Ok 2024-01-20T22:02:01Z 2024-01-20T22:02:01Z 2021-09-03 2009-02 0163-3864 https://pubs.kist.re.kr/handle/201004/132768 The nutrient conditions present in abandoned coal mine drainages create an extreme environment where defensive and offensive microbial interactions could be critical for survival and fitness. Coculture of a mine drainage-derived Sphingomonas bacterial strain, KMK-001, and a mine drainage-derived Aspergillus fumigatus fungal strain, KMC-901, resulted in isolation of a new diketopiperazine disulfide, glionitrin A (1). Compound I was not detected in monoculture broths of KMK-001 or KMC-901. The structure of 1, a (3S,10aS) diketopiperazine disulfide containing a nitro aromatic ring, was based on analysis of MS, NMR, and circular dichroism spectra and confirmed by X-ray crystal data. Glionitrin A displayed significant antibiotic activity against a series of microbes including methicillin-resistant Staphylococcus aureus. An in vitro MTT cytotoxicity assay revealed that I had potent submicromolar cytotoxic activity against four human cancer cell lines: HCT-116, A549, AGS, and DU145. The results provide further evidence that microbial coculture can produce novel biologically relevant molecules. English AMER CHEMICAL SOC CELL CYCLE INHIBITORS ASPERGILLUS-FUMIGATUS BACTERIUM FUNGUS DEGRADATION ACID COCULTIVATION SPORIDESMINS INDUCTION GLIOTOXIN Glionitrin A, an Antibiotic-Antitumor Metabolite Derived from Competitive Interaction between Abandoned Mine Microbes Article 10.1021/np800606e 1 JOURNAL OF NATURAL PRODUCTS, v.72, no.2, pp.248 - 252 JOURNAL OF NATURAL PRODUCTS 72 2 248 252 scie scopus 000263768400012 2-s2.0-62649098307 Plant Sciences Chemistry, Medicinal Pharmacology & Pharmacy Plant Sciences Pharmacology & Pharmacy Article CELL CYCLE INHIBITORS ASPERGILLUS-FUMIGATUS BACTERIUM FUNGUS DEGRADATION ACID COCULTIVATION SPORIDESMINS INDUCTION GLIOTOXIN Co-Culture Mine Microorganisms Sphingomonas Aspergillus Novel Compound anticancer antibiotics Glionitrin