Falk, Michael W. Song, Kyung-Guen Matiasek, Michael G. Wuertz, Stefan 2024-01-20T22:02:32Z 2024-01-20T22:02:32Z 2021-09-01 2009-02 0043-1354 https://pubs.kist.re.kr/handle/201004/132788 We operated 4 replicate membrane bioreactors (MBRs) in parallel to test if an acclimated seed inoculum would evolve similarly following even distribution into replicates. A cloning and sequencing library of 16S rRNA genes was obtained from the seed inoculum complemented with terminal restriction fragment length polymorphism (T-RFLP; n = 18 per reactor) analysis over the study period (n = 113 d) that targeted the 16S rRNA gene. The amoA functional gene was also monitored by T-RFLP. The T-RFLP results were analyzed by means of diversity indices, an adaptation of a moving window of similarity approach within each MBR, and non-metric multi-dimensional scaling (NMS) accompanied with multi-response permutation procedures (MRPP) to assess community interrelationships amongst MBRs. Based on the 16S rRNA microbial communities, the 4 MBRs initially diverged away from one another, followed by a convergence on Day 4. From thereon, the 16S rRNA-based communities evolved similarly throughout (average p-value = 0.49 from pair-wise MRPP). In contrast, the nitrifying communities did not undergo any discernable shift over time amongst MBRs according to T-RFLP analysis of amoA and revealed one cluster by NMS (average p-value = 0.83 from pair-wise MRPP). The study demonstrates that acclimated microbial communities evolve similarly over time in engineered systems when operational parameters are left unchanged. (C) 2008 Published by Elsevier Ltd. English PERGAMON-ELSEVIER SCIENCE LTD RESTRICTION-FRAGMENT-LENGTH WASTE-WATER TREATMENT 16S RIBOSOMAL-RNA AMMONIA-OXIDIZING BACTERIA NEIGHBOR-JOINING METHOD ACTIVATED-SLUDGE POPULATION-DYNAMICS SPECIES RICHNESS RFLP ANALYSIS DIVERSITY Microbial community dynamics in replicate membrane bioreactors - Natural reproducible fluctuations Article 10.1016/j.watres.2008.11.021 1 WATER RESEARCH, v.43, no.3, pp.842 - 852 WATER RESEARCH 43 3 842 852 scie scopus 000263658700030 Engineering, Environmental Environmental Sciences Water Resources Engineering Environmental Sciences & Ecology Water Resources Article RESTRICTION-FRAGMENT-LENGTH WASTE-WATER TREATMENT 16S RIBOSOMAL-RNA AMMONIA-OXIDIZING BACTERIA NEIGHBOR-JOINING METHOD ACTIVATED-SLUDGE POPULATION-DYNAMICS SPECIES RICHNESS RFLP ANALYSIS DIVERSITY Membrane bioreactor T-RFLP Microbial community dynamics Ammonia oxidizing bacteria 16S rRNA genes