Cho, Hye-Jin Kim, Jong Dae Lee, Won-Yong Chung, Bong Chul Choi, Man Ho 2024-01-20T22:02:44Z 2024-01-20T22:02:44Z 2021-09-01 2009-01-19 0003-2670 https://pubs.kist.re.kr/handle/201004/132795 Since corticosteroid metabolism may be affected by disease states, the accurate and precise measurement of endogenous corticosteroids in urine is necessary to understand their biochemical roles. An efficient quantitative profiling of 21 endogenous corticosteroids in urine has been validated by liquid chromatography-tandem mass spectrometry (LC-MS/MS). After enzymatic hydrolysis with beta-glucuronidase, samples were purified using a solid-phase extraction cartridge and then separated through a sub-2 mu m particle C18 column (2.1 mm x 50 mm, 1.9 mu m) and quantified within 12.1 min using a triple quadrupole MS with electrospray ionization in positive ion mode. All corticosteroids resulted in the base-line separation, which is even achieved for stereo-isomers, such as alpha-/beta-cortol, alpha-/beta-cortolone, and allo-tetrahydrocortisol/tetrahydrocortisol. Overall recoveries ranged from 85% to 106% with limit of quantification ranged from 0.5 to 2.0 ng mL(-1) for the corticosteroids examined. The precision (% CV) and accuracy (% bias) of the assay were 1.7-7.8% and 95.1-105.4%, respectively, in 0.5-200 ng mL(-1) calibration ranges (r(2) > 0.9903), for quality-control samples containing 21 endogenous corticosteroids at three different urinary concentrations. Clinical application included quantitative analysis from patients with both prostate cancer and benign prostatic hyperplasia with altered cortisol concentrations. The described LC-MS/MS method eliminates interference from other urine components, has excellent chromatographic resolution achieved by a small particle LC column with a sufficient sensitivity to allow the profiling of both gluco- and mineralo-corticosteroids at a time. (C) 2008 Elsevier B.V. All rights reserved. English ELSEVIER ALTERED CORTISOL METABOLISM QUANTIFICATION STEROIDS MINERALOCORTICOIDS INFLAMMATION EXTRACTION PATTERNS PLASMA SALT RAT Quantitative metabolic profiling of 21 endogenous corticosteroids in urine by liquid chromatography-triple quadrupole-mass spectrometry Article 10.1016/j.aca.2008.10.059 1 ANALYTICA CHIMICA ACTA, v.632, no.1, pp.101 - 108 ANALYTICA CHIMICA ACTA 632 1 101 108 scie scopus 000262585500014 2-s2.0-57649094408 Chemistry, Analytical Chemistry Article ALTERED CORTISOL METABOLISM QUANTIFICATION STEROIDS MINERALOCORTICOIDS INFLAMMATION EXTRACTION PATTERNS PLASMA SALT RAT Corticosteroids Liquid chromatography Tandem mass spectrometry Prostate diseases