Kim, Hyoung Ja Lee, Jun Young Kim, Sung Min Park, Da-Ae Jin, Changbae Hong, Seon Pyo Lee, Yong Sup 2024-01-20T22:04:11Z 2024-01-20T22:04:11Z 2021-09-03 2009-01 0367-326X https://pubs.kist.re.kr/handle/201004/132853 Calpains are calcium-dependent proteases that cleave a variety of intracellular substrates. The overactivation of mu-calpain is associated with a wide range of disease conditions. To search for calpain inhibitors from natural products, the phytochemical constituents of the ethyl acetate fraction of the whole plant of Orostachys japonicus (Crassulaceae) were studied. The various chromatographic separation of this fraction led to the isolation of a new tannin, (-)-epicatechin 5-gallate (1) along with 9 known compounds. Their structures were elucidated by spectroscopic and chemical analyses. Among them, (-)-epicatechin 5-gallate (1) and kaempferol (9) exhibited moderate inhibitory activity against mu-calpain with IC50 values of 18.0 +/- 2.9 and 15.4 +/- 2.0 mu g/ml, respectively. (C) 2008 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV PHENOLIC-COMPOUNDS SYSTEM A new epicatechin gallate and calpain inhibitory activity from Orostachys japonicus Article 10.1016/j.fitote.2008.10.003 1 FITOTERAPIA, v.80, no.1, pp.73 - 76 FITOTERAPIA 80 1 73 76 scie scopus 000263215600018 2-s2.0-58149099943 Chemistry, Medicinal Pharmacology & Pharmacy Pharmacology & Pharmacy Article PHENOLIC-COMPOUNDS SYSTEM Orostachys japonicus Crassulaceae mu-Calpain (-)-Epicatechin 5-gallate