Woo, Seonock Son, Sung Hee Park, Hong-Seog Vulpe, Chris D. Ryu, Jae-Chun Yum, Seungshic 2024-01-20T22:04:29Z 2024-01-20T22:04:29Z 2021-09-03 2008-12-31 1738-642X https://pubs.kist.re.kr/handle/201004/132863 To screen the differentially expressed genes in cadmuim-exposed marine medaka fish (Oryzias javanicus), a candidate marine test fish for ecological toxicity, the differential display polymerase chain reaction (DD-PCR) was carried out, since the genome-wide gene expression data are not available in this fish species yet. A total of 35 clones were isolated from cadmium-exposed fish and their nucleotide sequences were analyzed. The differentially expressed gene candidates were categorized to response to stimulus (3); ion binding (3); DNA binding (1); protein binding (6); carbohydrate binding (1); metabolic process (4); biological regulation (3); cellular process (2); protein synthesis (2); catalytic activity (2); sense of sight (1); immune (1); neurohormone (1); signaling activity (1); electron carrier activity (1) and others (3). For real-time quantitative RT-PCR, we selected catalase, glucose-6-phosphate dehydrogenase, heat shock protein 70, and metallothionein and confirmed that cadmium exposure enhanced induction of these four genes. English KOREAN SOCIETY TOXICOGENOMICS & TOXICOPROTEOMICS-KSTT TROUT SALMO-TRUTTA OXIDATIVE STRESS JAVA-MEDAKA ANTIOXIDANT ENZYMES MESSENGER-RNA INDUCTION METALLOTHIONEIN BIOMARKERS DISPLAY CELLS Differentially Expressed Genes in Marine Medaka Fish (Oryzias javanicus) Exposed to Cadmium Article 1 MOLECULAR & CELLULAR TOXICOLOGY, v.4, no.4, pp.293 - 299 MOLECULAR & CELLULAR TOXICOLOGY 4 4 293 299 scie kci other ART001465564 000262121900004 Biochemistry & Molecular Biology Toxicology Biochemistry & Molecular Biology Toxicology Article TROUT SALMO-TRUTTA OXIDATIVE STRESS JAVA-MEDAKA ANTIOXIDANT ENZYMES MESSENGER-RNA INDUCTION METALLOTHIONEIN BIOMARKERS DISPLAY CELLS Oryzias javanicus Cadmium Differential gene expression Biomarker Environmental stress