Kim, Jin Young Cheong, Jae Chul Ko, Beom Jun Lee, Sang Kyu Yoo, Hye Hyun Jin, Changbae In, Moon Kyo 2024-01-20T22:06:36Z 2024-01-20T22:06:36Z 2021-09-03 2008-12 0253-6269 https://pubs.kist.re.kr/handle/201004/132949 A liquid chromatography-electrospray ionization-tandem mass spectrometric (LC-ESI-MS/MS) method was developed and validated for the simultaneous detection and quantification of seven amphetamine derivatives (amphetamine (AP), methamphetamine (MA), 3,4-methylenedioxy-N-amphetamine (MDA), 3,4-methylenedioxy-N-methamphetamine (MDMA), 3,4-methylenedioxy-N-ethylamphetamine (MDEA), N,N-dimethylamphetamine (DMA), and N,N-dimethylamphetamine-N-oxide (DMANO)) in human urine. Seven deuterium-labeled compounds were prepared for use as internal standards to quantify the analytes. One milliliter of urine was combined with 1 mL of 0.2 M sodium carbonate buffer solution (pH 9.0) before solid phase extraction (SPE). An Oasis HLB SPE column followed by chromatographic separation on a Capcell Pak C18 MG-II column (150 x 2.0 mm I.D., 5 mu m) and electrospray mass spectrometry with multiple reaction monitoring were used for selective and sensitive detection. The use of ammonium formate (5 mM, pH adjusted to 4.0 with formic acid, Solvent A) and acetonitrile (Solvent B) as the mobile phase at a flow rate of 230 mu L/min was found to be the most effective for the separation. The linear ranges were 5.0-1000 ng/mL for AP, MDA, MDMA, MDEA, DMA, and DMANO and 10.0-1000 ng/mL for MA, with good correlation coefficients (r(2) > 0.996). The intra-day, inter-day, and interperson precisions were within 14.6%, 12.1% and 15.5%, respectively. The intra-day, inter-day, and interperson accuracies were between -11.6 and 9.0%, -7.9 and 2.3%, and -13.2 and 4.3%, respectively. The limits of detection (LODs) for each analytical compound were lower than 1.95 ng/mL. The recovery ranged from 72.3 to 103.3%. The applicability of the developed method was examined by analyzing several urine samples from confirmed drug abusers. English PHARMACEUTICAL SOC KOREA DIMETHYLAMPHETAMINE IDENTIFICATION AMPHETAMINE INJECTION Simultaneous determination of methamphetamine, 3,4-methylenedioxy-N-methylamphetamine, 3,4-methylenedioxy-N-ethylamphetamine, N,N-dimethylamphetamine, and their metabolites in urine by liquid chromatography-electrospray ionization-tandem mass spectrometry Article 10.1007/s12272-001-2163-5 1 ARCHIVES OF PHARMACAL RESEARCH, v.31, no.12, pp.1644 - 1651 ARCHIVES OF PHARMACAL RESEARCH 31 12 1644 1651 scie scopus kci ART001296667 000261938900019 2-s2.0-58449126358 Chemistry, Medicinal Pharmacology & Pharmacy Pharmacology & Pharmacy Article DIMETHYLAMPHETAMINE IDENTIFICATION AMPHETAMINE INJECTION Methamphetamine Dimethylamphetamine MDMA MDEA LC-MS/MS Urine