Cha, Kwang Hyun Koo, Song Yi Lee, Dong-Un 2024-01-20T22:30:33Z 2024-01-20T22:30:33Z 2021-09-03 2008-11-26 0021-8561 https://pubs.kist.re.kr/handle/201004/132972 The antiproliferative activity of carotenoids separated from marine Chlorella ellipsoidea and freshwater Chlorella vulgaris has been evaluated. HPLC analysis revealed that the main carotenoid from C. ellipsoidea was composed of violaxanthin with two minor xanthophylls, antheraxanthin and zeaxanthin, whereas the carotenoid from C. vulgaris was almost completely composed of lutein. In an MTT assay, both semipurified extracts of C. ellipsoidea and C. vulgaris inhibited HCT116 cell growth in a dose-dependent manner, yielding IC50 values of 40.73 +/- 3.71 and 40.31 +/- 4.43 mu g/mL, respectively. In addition, treatment with both chlorella extracts enhanced the fluorescence intensity of the early apoptotic cell population in HCT116 cells. C. ellipsoidea extract produced an apoptosis-inducing effect almost 2.5 times stronger than that of the C. vulgaris extract. These results indicate that bioactive xanthophylls of C. ellipsoidea might be useful functional ingredients in the prevention of human cancers. English AMER CHEMICAL SOC LIQUID-CHROMATOGRAPHY INDUCED APOPTOSIS LUTEIN MICROALGAE ACCUMULATION PYRENOIDOSA ASTAXANTHIN NEOXANTHIN ACTIVATION SEPARATION Antiproliferative Effects of Carotenoids Extracted from Chlorella ellipsoidea and Chlorella vulgaris on Human Colon Cancer Cells Article 10.1021/jf802111x 1 JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.56, no.22, pp.10521 - 10526 JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 56 22 10521 10526 scie scopus 000261056700014 2-s2.0-57849131877 Agriculture, Multidisciplinary Chemistry, Applied Food Science & Technology Agriculture Chemistry Food Science & Technology Article LIQUID-CHROMATOGRAPHY INDUCED APOPTOSIS LUTEIN MICROALGAE ACCUMULATION PYRENOIDOSA ASTAXANTHIN NEOXANTHIN ACTIVATION SEPARATION Chlorella ellipsoidea Chlorella vulgaris carotenoid antiproliferative effects apoptosis