Lee, Sanghyun Park, Hee-Seung Notsu, Yohko Ban, Hyun Seung Kim, Yong Pil Ishihara, Kenji Hirasawa, Noriyasu Jung, Sang Hoon Lee, Yeon Sil Lim, Soon Sung Park, Eun-Hee Shin, Kuk Hyun Seyama, Toshio Hong, JangJa Ohuchi, Kazuo 2024-01-20T22:31:36Z 2024-01-20T22:31:36Z 2021-09-03 2008-11 0951-418X https://pubs.kist.re.kr/handle/201004/133013 The extract of the root of Acanthopanax chiisanensis Nakai is used for the treatment of inflammation. To analyse the action mechanism of this extract, the effect of hyperin (quercetin-3-O-beta-D-galactose) isolated from the ethyl acetate fraction of the root of A. chiisanensis on nitrite production and induction of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS, 1 mu g/mL)-stimulated rat peritoneal macrophages were examined. The effect of the structurally related compounds, isoquercitrin (quercetin-3-O-beta-glucose) and quercetin (an aglycone of the two compounds) isolated from the extract of the leaves of Vaccinium koreanum Nakai was also examined to compare the effect. It was shown that hyperin inhibited the LPS-induced iNOS expression and nitrite production. Of the three compounds, quercetin showed the most potent inhibitory activity. The phosphorylation of p44/42 mitogen activated protein kinase (MAPK), p38 MAPK and c-Jun N-terminal kinase (JNK) were also inhibited by these compounds. These findings suggested that hyperin in the extract of the root of A. chiisanensis inhibits nitric oxide (NO) production through inhibition of the expression of iNOS by attenuation of p44/p42 MAPK, p38 MAPK and JNK, and thus participates in the antiinflammatory activity of the extract. Copyright (C) 2008 John Wiley & Sons, Ltd. English WILEY PROSTAGLANDIN E-2 PRODUCTION ACANTHOPANAX-CHIISANENSIS OXIDE PRODUCTION APICULAREN-A INDUCTION INHIBITION APOPTOSIS TECTORIGENIN ISOFLAVONES TECTORIDIN Effects of Hyperin, Isoquercitrin and Quercetin on Lipopolysaccharide-induced Nitrite Production in Rat Peritoneal Macrophages Article 10.1002/ptr.2529 1 PHYTOTHERAPY RESEARCH, v.22, no.11, pp.1552 - 1556 PHYTOTHERAPY RESEARCH 22 11 1552 1556 scie scopus 000261124900025 2-s2.0-57349117995 Chemistry, Medicinal Pharmacology & Pharmacy Pharmacology & Pharmacy Article PROSTAGLANDIN E-2 PRODUCTION ACANTHOPANAX-CHIISANENSIS OXIDE PRODUCTION APICULAREN-A INDUCTION INHIBITION APOPTOSIS TECTORIGENIN ISOFLAVONES TECTORIDIN Acanthopanax chiisanensis nitric oxide (NO) hyperin isoquercitrin quercetin macrophages