Islam, M. Nurul Yoo, Hye Hyun Lee, Jun Nam, Joo Won Seo, Eun Kyoung Jin, Changbae Kim, Dong-Hyun 2024-01-20T22:31:51Z 2024-01-20T22:31:51Z 2021-09-03 2008-11 1060-3271 https://pubs.kist.re.kr/handle/201004/133023 The rhizomes of Anemarrhena asphodeloides Bunge (Liliaceae) are prescribed as crude drugs in herbal medication for the treatment of various diseases such as diabetes, inflammation, and platelet aggregation inhibition. A simple, sensitive, and precise reversed-phase liquid chromatographic method was developed to study the quantitative determination of 5 bioactive compounds from these rhizomes, namely, neomangiferin, mangiferin, isomangiferin, nyasol, and methylnyasol. Chromatographic analysis was performed on Capcell Pak C-18 column (150 x 4.6 mm, 3 mu m) with a mobile phase consisting of acetonitrile, methanol, and 0.1% formic acid at a flow rate of 1.00 mL/min. Quantitation was performed using a UV-visible detector at 260 nm. The method for the determination of reported medicinal agents was accurate and reproducible. Excellent linear behavior was observed over the investigated concentration range of 2.5-100.0 mu g/mL for neomangiferin; 1.5-60.0 mu g/mL for mangiferin; 0.5-20.0 mu g/mL for nyasol; and 0.2-20.0 mu g/mL for methylnyasol; correlation coefficient > 0.99. The intraday and interday precision over the concentration range of compounds was < 6.6% (relative standard deviation) and accuracy was between 94.9 and 109.3%. This method can be successfully applied for the analysis of medicinal compounds from the ethanolic extract of A. asphodeloides Bunge. English AOAC INT TANDEM MASS-SPECTROMETRY SWERTIA-MUSSOTII FRANCH RAT PLASMA CAPILLARY-ELECTROPHORESIS ANTIDIABETIC ACTIVITY HYPOGLYCEMIC ACTIVITY ACTIVE CONSTITUENTS MANGIFERIN IDENTIFICATION DIFFERENTIATION Simultaneous Determination of Bioactive Xanthone Glycosides and Norlignans from Ethanolic Extract of Anemarrhena asphodeloides by Liquid Chromatography Article 1 JOURNAL OF AOAC INTERNATIONAL, v.91, no.6, pp.1271 - 1277 JOURNAL OF AOAC INTERNATIONAL 91 6 1271 1277 scie scopus 000262118200006 2-s2.0-58449134302 Chemistry, Analytical Food Science & Technology Chemistry Food Science & Technology Article TANDEM MASS-SPECTROMETRY SWERTIA-MUSSOTII FRANCH RAT PLASMA CAPILLARY-ELECTROPHORESIS ANTIDIABETIC ACTIVITY HYPOGLYCEMIC ACTIVITY ACTIVE CONSTITUENTS MANGIFERIN IDENTIFICATION DIFFERENTIATION