Cho, Ik Sang Kim, Jung Lim, Do Hwan Ahn, Hee-Chul Kim, Hyunggee Lee, Kang-Bong Lee, Young Sik 2024-01-20T22:31:54Z 2024-01-20T22:31:54Z 2021-09-03 2008-11 0141-5492 https://pubs.kist.re.kr/handle/201004/133025 Small interfering RNAs (siRNAs) which mediate sequence-specific gene silencing through RNA interference have been harnessed for therapeutic applications. Here, we found combinations of modest 2'-ribose siRNA modifications that permit effective gene silencing and improve siRNA stability in human serum. Introduction of as few as two different or identical 2'-ribose modifications at a specific position of either strand, which has been previously suggested to suppress unintended off-target effects, enhances the thermal and thermodynamic stability of siRNA duplexes, and does not alter the overall A-form helical geometry that is required for silencing activity. Our results provide a useful template for the design for therapeutic siRNAs. English SPRINGER SMALL INTERFERING RNA FUNCTIONAL FEATURES STRUCTURAL BASIS DEGRADATION RECOGNITION Improved serum stability and biophysical properties of siRNAs following chemical modifications Article 10.1007/s10529-008-9776-4 1 BIOTECHNOLOGY LETTERS, v.30, no.11, pp.1901 - 1908 BIOTECHNOLOGY LETTERS 30 11 1901 1908 scie scopus 000259412200004 2-s2.0-52449103310 Biotechnology & Applied Microbiology Biotechnology & Applied Microbiology Article SMALL INTERFERING RNA FUNCTIONAL FEATURES STRUCTURAL BASIS DEGRADATION RECOGNITION A-form helix melting temperature 2 &apos -ribose modification RNA interference siRNA stability