Lee, Soo Hyun Kim, Sung-Woo Kang, Ji Yoon Ahn, Chong H. 2024-01-20T22:32:08Z 2024-01-20T22:32:08Z 2021-09-02 2008-11 1473-0197 https://pubs.kist.re.kr/handle/201004/133034 An innovative polymer lab-on-a-chip (LOC) for reverse transcription (RT)-polymerase chain reaction (PCR) has been designed, fabricated, and characterized for point-of-care testing (POCT) clinical diagnostics. In addition, a portable analyzer that consists of a non-contact infrared (IR) based temperature control system for RT-PCR process and an optical detection system for on-chip detection, has also been developed and used to monitor the RT-PCR LOC. The newly developed LOC and analyzer have been interfaced and optimized for performing RT-PCR procedures and chemiluminescence assays in sequence. As a clinical diagnostic application, human immunodeficiency virus (HIV) for the early diagnosis of acquired immune deficiency syndrome (AIDS) has been successfully detected and analyzed using the newly developed LOC and analyzer, where the primer sets for p24 and gp120 were used as the makers for HIV. The developed polymer LOC and analyzer for RTPCR can be used for POCT for the analysis of HIV with the on-chip RT-PCR and chemiluminescence assays in shorter than one hour with minimized cross-contamination. English ROYAL SOC CHEMISTRY CHAIN-REACTION AMPLIFICATION DNA AMPLIFICATION PCR DEVICE SYSTEM BIOCHIP VALVES A polymer lab-on-a-chip for reverse transcription (RT)-PCR based point-of-care clinical diagnostics Article 10.1039/b811131f 1 LAB ON A CHIP, v.8, no.12, pp.2121 - 2127 LAB ON A CHIP 8 12 2121 2127 scie scopus 000261686200017 2-s2.0-56549102421 Biochemical Research Methods Chemistry, Multidisciplinary Chemistry, Analytical Nanoscience & Nanotechnology Instruments & Instrumentation Biochemistry & Molecular Biology Chemistry Science & Technology - Other Topics Instruments & Instrumentation Article CHAIN-REACTION AMPLIFICATION DNA AMPLIFICATION PCR DEVICE SYSTEM BIOCHIP VALVES PCR