Lee, Sang Kyu Lee, Dong Ju Yoo, Hye Hyun Kim, Ju Hyun Seo, Young Nun Shin, Sil Choi, Jae Ho Jeon, Tae Won Kang, Mi Jeong Jeong, Tae Cheon 2024-01-20T22:33:55Z 2024-01-20T22:33:55Z 2021-09-03 2008-10 0253-6269 https://pubs.kist.re.kr/handle/201004/133103 Halogenated organic compounds, such as 1-bromopentane (1-BPT), are used as cleaning agents, synthesis agents, or extraction solvents in the workplace. In the present study, glutathione (GSH) conjugation and hepatotoxicity induced by 1-BPT were investigated in female BALB/c mice. S-Bromopentyl GSH, S-bromopentyl cysteine, and mono-hydroxypentyl mercapturic acid were identified in liver by liquid chromatography-electrospray ionization tandem mass spectrometry. Oral treatment of mice with 1-BPT at 1500 mg/kg produced maximum GSH conjugates at 6 h after treatment. For hepatotoxicity tests, the animals were treated orally with 1-BPT at 375, 750, or 1500 mg/kg in corn oil once for a dose response study or at 1500 mg/kg for 6, 12, 24, or 48 h for a time course study. 1-BPT dose-dependently increased serum activity of ALT and AST and decreased hepatic GSH levels, peaking at 6 and 12 h after treatment. 1-BPT (750 and 1500 mg/kg) also significantly increased the hepatic content of malondialdehyde. Thus, 1-BPT could cause hepatotoxicity and depletion of GSH content by forming GSH conjugates, presenting a toxicity mechanism and potential biomarkers for low molecular weight haloalkanes. English PHARMACEUTICAL SOC KOREA TANDEM MASS-SPECTROMETRY RAT 1,2-DIBROMOPROPANE METABOLISM ACIDS Identification of Glutathione Conjugates of 1-Bromopentane and its Hepatotoxicity in Female BALB/c Mice Article 10.1007/s12272-001-2112-3 1 ARCHIVES OF PHARMACAL RESEARCH, v.31, no.10, pp.1317 - 1323 ARCHIVES OF PHARMACAL RESEARCH 31 10 1317 1323 scie scopus kci 000260302300013 2-s2.0-55149124042 Chemistry, Medicinal Pharmacology & Pharmacy Pharmacology & Pharmacy Article TANDEM MASS-SPECTROMETRY RAT 1,2-DIBROMOPROPANE METABOLISM ACIDS 1-Bromopentane Glutathione Conjugate structure Hepatotoxicity In vivo Mice