Kim, Youn-Jung Song, Mi-Kyung Sarma, Sailendra Nath Choi, Han-Saem Ryu, Jae-Chun 2024-01-20T22:34:41Z 2024-01-20T22:34:41Z 2021-09-03 2008-09-30 1738-642X https://pubs.kist.re.kr/handle/201004/133138 Volatile organic compounds (VOCs) are common constituents of cleaning and degreasing agents, paints, pesticides, personal care products, gasoline and solvents. And VOCs are evaporated at room temperature and most of them exhibit acute and chronic toxicity to human. Benzene is the most widely used prototypical VOC and the toxic mechanisms of them are still unclear. The multi-step process of toxic mechanism can be more fully understood by characterizing gene expression changes induced in cells by toxicants. In this study, DNA microarray was used to monitor the expression levels of genes in HepG2 cells and HL-60 cells exposed to the benzene on IC20 and IC50 dose respectively. In the clustering analysis of gene expression profiles, although clusters of HepG2 and HL-60 cells by benzene were divided differently, expression pattern of many genes observed similarly. We identified 916 up-regulated genes and 1,144 down-regulated genes in HepG2 cells and also 1,002 up-regulated genes and 919 down-regulated genes in HL-60 cells. The gene ontology analysis on genes expressed by benzene in and HL-60 cells, respectively, was performed. Thus, we found some principal pathways, such as, focal adhesion, gap junction and signaling pathway in HepG2 cells and toll-like receptor signaling pathway, MAPK signaling pathway, p53 signaling pathway and neuroactive ligand-receptor interaction in HL-60 cells. And we also found 16 up-regulated and 14 down-regulated commonly expressed total 30 genes that belong in the same biological process like inflammatory response, cell cycle arrest, cell migration, transmission of nerve impulse and cell motility in two cell lines. In conclusion, we suggest that this study is meaningful because these genes regarded as strong potential biomarkers of benzene independent of cell type. English KOREAN SOCIETY TOXICOGENOMICS & TOXICOPROTEOMICS-KSTT Functional gene analysis to identify potential markers induced by benzene in two different cell lines, HepG2 and HL-60 Article 1 MOLECULAR & CELLULAR TOXICOLOGY, v.4, no.3, pp.183 - 191 MOLECULAR & CELLULAR TOXICOLOGY 4 3 183 191 scie kci other ART001465537 000259633500002 Biochemistry & Molecular Biology Toxicology Biochemistry & Molecular Biology Toxicology Article volatile organic compounds (VOCs) benzene microarray gene ontology