Lee, Seulki Park, Kyeongsoon Lee, Seung-Young Ryu, Ju Hee Park, Jong Woong Ahn, Hyung Jun Kwon, Ick Chan Youn, In-Chan Kim, Kwangmeyung Choi, Kuiwon 2024-01-20T22:36:09Z 2024-01-20T22:36:09Z 2021-09-03 2008-09 1043-1802 https://pubs.kist.re.kr/handle/201004/133208 The early detection of osteoarthritis (OA) is currently a key challenge in the field of rheumatology. Biochemical studies of OA have indicated that matrix metalloproteinase-13 (MMP-13) plays a central role in cartilage degradation. In this study, we describe the potential use of a dark-quenched fluorogenic MMP-13 probe to image MMP-13 in both in vitro and rat models. The imaging technique involved using a MMP-13 peptide substrate, near-infrared (NIR) dye, and a NIR dark quencher. The results from this study demonstrate that the use of a dark-quenched fluorogenic probe allows for the visual detection of MMP-13 in vitro and in OA-induced rat models. In particular, by targeting this OA biomarker, the symptoms of the early and late stages of OA can be readily monitored, imaged, and analyzed in a rapid and efficient fashion. We anticipate that this simple and highly efficient fluorogenic probe will assist in the clinical management of patients with OA, not only for early diagnosis but also to assess individual patient responses to new drug treatments. English AMER CHEMICAL SOC POLYMERIC NANOPARTICLES CARTILAGE EXPRESSION MMP-13 Dark quenched matrix metalloproteinase fluorogenic probe for imaging osteoarthritis development in vivo Article 10.1021/bc800264z 1 BIOCONJUGATE CHEMISTRY, v.19, no.9, pp.1743 - 1747 BIOCONJUGATE CHEMISTRY 19 9 1743 1747 scie scopus 000259358000001 2-s2.0-52249097640 Biochemical Research Methods Biochemistry & Molecular Biology Chemistry, Multidisciplinary Chemistry, Organic Biochemistry & Molecular Biology Chemistry Article POLYMERIC NANOPARTICLES CARTILAGE EXPRESSION MMP-13 imaging osteoarthritis mmp