Kim, Dongin Lee, Eun Seong Park, Kyeongsoon Kwon, Ick Chan Bae, You Han 2024-01-20T22:36:11Z 2024-01-20T22:36:11Z 2021-09-03 2008-09 0724-8741 https://pubs.kist.re.kr/handle/201004/133209 Purpose. To evaluate pH-sensitive mixed micelles for multidrug resistant (MDR) ovarian tumor targeting and optical imaging of solid tumors. Method Doxorubicin (DOX) encapsulated pH-sensitive mixed micelles composed Of poly(L-histidine) (MW 5K)-b-PEG(MW 2K) and poly(L-lactic acid)(3K)-b-PEG (2K)-folate (PHSM-f) were prepared. Folate receptor-mediated endocytosis, drug uptake, endosomal disruption and cell viability were investigated at the cellular level. For in vivo tumor growth inhibition tests, multidrug resistant ovarian A2780/DOXR xenografted nude mice were used. Optical imaging was performed by using a Cy5.5 fluorescence dye-labeled mixed micelle system. Cy5.5 fluorescence intensity at the tumor site was measured in KB epidermoid xenografted nude mice. Results. In vitro cell viability and drug distribution in the cytoplasm demonstrated the significantly superior efficacy of PHSM-f to free DOX and a control sample of DOX loaded pH-insensitive micelle composed of poly(L-lactic acid)(3K)-b-PEG(2K)/poly(L-lactic acid)(3K)-b-PEG(2K)-folate (80/20 wt/wt%) (PHIM-f). The mechanisms of these results were proved by folate receptor mediated endocytosis of micelle and endosomal disruption function by it. In addition, the optical imaging demonstrated the future application of the diagnositic area. PHSM-f inhibited the growth of multidrug resistant ovarian tumors efficiently in mice, with minimum weight loss. Conclusions. The pH-sensitive mixed micelle system demonstrates effective antitumor efficacy against the multidrug resistant ovarian tumor A2780/DOXR. English SPRINGER/PLENUM PUBLISHERS BLOCK-COPOLYMER MICELLES DRUG-DELIVERY POLYMERIC MICELLES SUPRAMOLECULAR ASSEMBLIES CANCER FOLATE DESIGN RESISTANCE RELEASE Doxorubicin Loaded pH-sensitive Micelle: Antitumoral Efficacy against Ovarian A2780/DOXR Tumor Article 10.1007/s11095-008-9603-6 1 PHARMACEUTICAL RESEARCH, v.25, no.9, pp.2074 - 2082 PHARMACEUTICAL RESEARCH 25 9 2074 2082 scie scopus 000258408400011 2-s2.0-49549098607 Chemistry, Multidisciplinary Pharmacology & Pharmacy Chemistry Pharmacology & Pharmacy Article BLOCK-COPOLYMER MICELLES DRUG-DELIVERY POLYMERIC MICELLES SUPRAMOLECULAR ASSEMBLIES CANCER FOLATE DESIGN RESISTANCE RELEASE folic acid micelle multidrug resistance ovarian cancer poly(L-histidine)