Na, Dae Seok Lee, Hwang Kim, Sun Uk Hwang, Chang Nam Lee, Sang Ho Kang, Ji Yoon Kim, Jai Kyeong Pak, James Jungho 2024-01-20T23:03:01Z 2024-01-20T23:03:01Z 2021-09-03 2008-07 0885-3282 https://pubs.kist.re.kr/handle/201004/133358 Various materials including glass and polymers have been widely used for stem cell culture due to their biocompatibility. However, the roles of these materials are fundamentally limited because they cannot realize or imitate the complex biological functions of living tissues, except in very simple cases. Here, the development of a bio-derived material suitable for stem cell culture and improvement of differentiation efficiency to specific cell lineages with no stimulating agents by using a chorion obtained from a fertilized zebrafish egg through the removal of the yolk and embryonic cell mass from the egg is reported. Mouse P19 EC stem cells introduced into the empty chorion form a uniform embryoid body (EB) without addition of any inducing agent. It is demonstrated that the zebrafish chorion with nanopores improves efficiencies greatly in the EB formation, cell proliferation, and lineage-specific differentiations compared to those of the conventional hanging drop culture method. English SAGE PUBLICATIONS LTD EMBRYONAL CARCINOMA-CELLS IN-VITRO EGG CHORION CULTURE SYSTEM EXPRESSION PROTEIN GATA-4 Comparative study of P19 EC stem cell differentiation in between conventional hanging drop and the zebrafish chorion as a bio-derived material Article 10.1177/0885328207084746 1 JOURNAL OF BIOMATERIALS APPLICATIONS, v.23, no.1, pp.73 - 84 JOURNAL OF BIOMATERIALS APPLICATIONS 23 1 73 84 scie scopus 000257326600004 2-s2.0-45749159036 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article EMBRYONAL CARCINOMA-CELLS IN-VITRO EGG CHORION CULTURE SYSTEM EXPRESSION PROTEIN GATA-4 P19 EC stem cell differentiation comparison hanging drop zebrafish chorion a bio-derived material