Kim, Youn-Jung Kim, Mi-Soon Jeon, Hee-Kyung Ryu, Jae-Chun 2024-01-20T23:03:26Z 2024-01-20T23:03:26Z 2021-09-03 2008-06-30 1738-642X https://pubs.kist.re.kr/handle/201004/133378 Methylmercury (MeHg) is known to have devastating effects on the mammalian nervous system. In order to characterize the mechanism of MeHg-induced neurotoxicity, we investigated the analysis of transcriptional profiles on human 8k cDNA microarray by treatment of 1.4 mu M MeHg at 3, 12, 24 and 48 h in human neuroblastoma SH-SY5Y cell line. Some of the identified genes by MeHg treatment were significant at early time points (3 h), while that of others was at late time points (48 h). The early response genes that may represent those involved directly in the MeHg response included pantothenate kinase 3, a kinase (PRKA) anchor protein (yotiao) 9, neurotrophic tyrosine kinase, receptor, type 2 gene, associated with NMDA receptor activity regulation or perturbations of central nervous system homeostasis. Also, when SH-SY5Y cells were subjected to a longer exposure (48 h), a relative increase was noted in a gene, glutamine-fructose-6-phosphate transaminase 1, reported that overexpression of this gene may lead to the increased resistance to MeHg. To confirm the alteration of these genes in cultured neurons, we then applied real time-RT PCR with SYBR green. Thus, this result suggests that a neurotoxic effect of the MeHg might be ascribed that MeHg alters neuronal receptor regulation or homeostasis of neuronal cells in the early phase. However, in the late phase, it protects cells from neurotoxic effects of MeHg. English KOREAN SOC TOXICOGENOMICS & TOXICOPROTEOMICS SELENOPROTEIN-W SACCHAROMYCES-CEREVISIAE TARGET BRAIN GLUTATHIONE RECEPTOR BINDING PROTEIN METALS YOTIAO Identification of genes associated with early and late response of methylmercury in human neuroblastoma cell line Article 1 MOLECULAR & CELLULAR TOXICOLOGY, v.4, no.2, pp.164 - 169 MOLECULAR & CELLULAR TOXICOLOGY 4 2 164 169 scie kci other ART001465531 000257266700011 Biochemistry & Molecular Biology Toxicology Biochemistry & Molecular Biology Toxicology Article SELENOPROTEIN-W SACCHAROMYCES-CEREVISIAE TARGET BRAIN GLUTATHIONE RECEPTOR BINDING PROTEIN METALS YOTIAO methylmercury human neuroblastoma cells transcriptional profile neurotoxic effect time responsive genes