Kang, Sun-Woong Yang, Hee Seok Seo, Sang-Woo Han, Dong Keun Kim, Byung-Soo 2024-01-20T23:03:53Z 2024-01-20T23:03:53Z 2021-09-03 2008-06-01 1549-3296 https://pubs.kist.re.kr/handle/201004/133400 Biodegradable polymer/ceramic composite scaffold could overcome limitations of biodegradable polymers or ceramics for bone regeneration. Injectable scaffold has raised great interest for bone regeneration in vivo, since it allows one for easy filling of irregularly shaped bone defects and implantation of osteogenic cells through minimally invasive surgical procedures The purpose of this study was to determine whether apatite-coated poly(lactic-co-glycolic acid) (PLGA) microspheres could be used as an injectable scaffold to regenerate bone in vivo. Apatite-coated PLGA microspheres were fabricated by incubating PLGA microspheres in simulated body fluid. The apatite that coated the PLGA microsphere surfaces was similar to apatite in natural bone, as demonstrated by scanning electron microscopy, X-ray diffraction spectra, energy-dispersive spectroscopy, and Fourier transformed-infrared spectroscopy analyses. Rat osteoblasts were mixed with apatite-coated PLGA microspheres and injected immediately into subcutaneous sites of athymic mice. Osteoblast transplantation with plain PLGA microspheres served as a control. Histological analysis of the implants at 6 weeks with hematoxylin and eosin staining, Masson's trichrome staining, and von Kossa staining revealed much better regeneration of bone in the apatite-coated PLGA microsphere group than the plain PLGA microsphere group. The new bone formation area and the calcium content of the implants were significantly higher in the apatite-coated PLGA microsphere group than in the plain PLGA microsphere group. This study demonstrates the feasibility of using apatite-coated PLGA microspheres as an injectable scaffold for in vivo bone tissue engineering. This scaffold may be useful for bone regeneration through minimally invasive surgical procedures in orthopedic applications. (C) 2007 Wiley Periodicals, Inc. English WILEY POTENTIAL BULKING AGENT COMPOSITE SCAFFOLDS IN-VITRO OSTEOBLAST ADHESION NANOPHASE CERAMICS BIOMIMETIC PROCESS CELL ATTACHMENT GLASS-CERAMICS REGENERATION CHONDROCYTES Apatite-coated poly(lactic-co-glycolic acid) microspheres as an injectable scaffold for bone tissue engineering Article 10.1002/jbm.a.31572 1 JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, v.85A, no.3, pp.747 - 756 JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 85A 3 747 756 scie scopus 000255530500022 2-s2.0-43049135223 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article POTENTIAL BULKING AGENT COMPOSITE SCAFFOLDS IN-VITRO OSTEOBLAST ADHESION NANOPHASE CERAMICS BIOMIMETIC PROCESS CELL ATTACHMENT GLASS-CERAMICS REGENERATION CHONDROCYTES bone regeneration biodegradable polymer polymer/ceramic composite injectable scaffold tissue engineering