Jeon, Daejong Chu, Kon Jung, Keun-Hwa Kim, Manho Yoon, Byung-Woo Lee, C. Justin Oh, Uhtaek Shin, Hee-Sup 2024-01-20T23:30:52Z 2024-01-20T23:30:52Z 2021-09-03 2008-05 0143-4160 https://pubs.kist.re.kr/handle/201004/133519 Na+/Ca2+ exchanger (NCX), by mediating Na+ and Ca2+, fluxes bi-directionally, assumes a role in controlling the Ca2+ homeostasis in the ischemic brain. It has been suggested that the three isoforms of NCX (NCX1, 2 and 3) may be differentially involved in permanent cerebral ischemia. However, the role of NCX2 has not been defined in ischemic reperfusion injury after a transient focal cerebral ischemia. Furthermore, it is not known whether NCX2 imports or exports intracellular Ca2+([Ca2+](i)) following ischemia and reperfusion. To define the role of NCX2 in ischemia and reperfusion, we examined mice lacking NCX2, in vivo and in vitro. After an in vitro ischemia, a significantly slower recovery in population spike amplitudes, a sustained elevation of [Ca2+](i) and an increased membrane depolarization were developed in the NCX2-deficient hippocampus. Moreover, a transient focal cerebral ischemia in vivo produced a larger infarction and more cell death in the NCX2-deficient mouse brain. In particular, in the wild type brain, NCX2-expressing neurons were largely spared from cell death after ischemia. Our results suggest that NCX2 exports Ca2+ in ischemia and thus protects neuronal cells from death by reducing [Ca2+](i) in the adult mouse brain. (c) 2007 Elsevier Ltd. All rights reserved. English ELSEVIER SCI LTD SODIUM-CALCIUM EXCHANGE NA+-CA2+ EXCHANGER BRAIN-REGIONS RAT-BRAIN IN-VITRO EXPRESSION NCX3 ISOFORMS NEURONS CLONING Na+/Ca2+ exchanger 2 is neuroprotective by exporting Ca2+ during a transient focal cerebral ischemia in the mouse Article 10.1016/j.ceca.2007.08.003 1 CELL CALCIUM, v.43, no.5, pp.482 - 491 CELL CALCIUM 43 5 482 491 scie scopus 000256325900006 2-s2.0-42149087628 Cell Biology Cell Biology Article SODIUM-CALCIUM EXCHANGE NA+-CA2+ EXCHANGER BRAIN-REGIONS RAT-BRAIN IN-VITRO EXPRESSION NCX3 ISOFORMS NEURONS CLONING cerebral ischemia Na+/Ca2+ exchanger NCX2-deficient mice [Ca2+](i) population spike membrane depolarization