Babu, C. V. Suresh Song, Eun Joo Babar, Sheikh Md. Enayetul Yoo, Young Sook 2024-01-20T23:31:25Z 2024-01-20T23:31:25Z 2022-01-10 2008-04-24 0165-022X https://pubs.kist.re.kr/handle/201004/133546 Phospholamban (PLN) is a key regulatory protein involved in cardiac calcium signaling through the pumping of cytoplasmic Ca(2+) into the sarcoplasmic reticulum (SR). Recent systems-level studies have focused on integrating quantitative data (e.g. protein expression levels) for a better understanding of cardiac systems biology. In this view, we developed a capillary electrophoresis (CE) based immunoprecipitation method for the measurement of phospho-PLN (ser 16) in cardiomyocytes (HL-1 cell line). Dose-dependent isoproterenol (Iso) treated cells were analyzed using CE, and the phospho-PLN levels were quantified using specific polyclonal antibodies. The CE method employed was accurate, quick and easier compare to other techniques and the results are useful for the subsequent computational systems biology research. (c) 2007 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV SUBSTANCE-P RAT-BRAIN ELECTROPHORESIS ANTIBODIES SITES Measurement of phosphorylated phospholamban levels in cardiomyocytes (HL-1) by immunoprecipitation Article 10.1016/j.jbbm.2007.05.008 1 JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, v.70, no.6, pp.838 - 844 JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS 70 6 838 844 scie scopus 000256393200007 2-s2.0-48649094069 Biochemical Research Methods Biochemistry & Molecular Biology Biophysics Biochemistry & Molecular Biology Biophysics Article SUBSTANCE-P RAT-BRAIN ELECTROPHORESIS ANTIBODIES SITES capillary electrophoresis Immunoprecipitation phospholamban HL-1