Kim, Jong-Ho Kim, Yoo-Shin Park, Kyeongsoon Lee, Seulki Nam, Hae Yun Min, Kyung Hyun Jo, Hyung Gon Park, Jae Hyung Choi, Kuiwon Jeong, Seo Young Park, Rang-Woon Kim, In-San Kim, Kwangmeyung Kwon, Ick Chan 2024-01-20T23:31:40Z 2024-01-20T23:31:40Z 2021-09-03 2008-04-07 0168-3659 https://pubs.kist.re.kr/handle/201004/133558 To make a tumor targeting nano-sized drug delivery system, biocompatible and biodegradable glycol chitosan (M-W=250 kDa) was modified with hydrophobic cholanic acid. The resulting hydrophobically modified glycol chitosans (HGCs) that formed nano-sized self-aggregates in an aqueous medium were investigated as an anticancer drug carrier in cancer treatment. Insoluble anticancer drug, cisplatin (CDDP), was easily encapsulated into the hydrophobic cores of HGC narroparticles by a dialysis method, wherein the drug loading efficiency was about 80%. The CCDP-encapsulated HGC (CDDP-HGC) nanoparticles were well-dispersed in aqueous media and they formed a nanoparticles structure with a mean diameter about 300-500 nm. As a nano-sized drug carrier, the CDDP-HGC nanoparticles released the drug in a sustained manner for a week and they were also less cytotoxic than was free CDDP, probably because of sustained release of CDDP from the HGC narroparticles. The tumor targeting ability of CDDP-HGC nanoparticles was confirmed by in vivo live animal imaging with near-infrared fluorescence Cy5.5-labeled CDDP-HGC nanoparticles. It was observed that CDDP-HGC nanoparticles were successfully accumulated by tumor tissues in tumor-bearing mice, because of the prolonged circulation and enhanced permeability and retention (EPR) effect of CDDP-HGC narroparticles in tumor-bearing mice. As expected, the CDDP-HGC nanoparticles showed higher antitumor efficacy and lower toxicity compared to free CDDP, as shown by changes in tumor volumes, body weights, and survival rates, as well as by immunohistological TUNEL assay data. Collectively, the present results indicate that HGC nanoparticles are a promising carrier for the anticancer drug CDDP. (C) 2008 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV SELF-ASSEMBLED NANOPARTICLES POLYMERIC MICELLES HPMA COPOLYMER DRUG-DELIVERY 5-BETA-CHOLANIC ACID CONTROLLED-RELEASE DOXORUBICIN CANCER CHEMOTHERAPY BIODISTRIBUTION Antitumor efficacy of cisplatin-loaded glycol chitosan nanoparticles in tumor-bearing mice Article 10.1016/j.jconrel.2007.12.014 1 JOURNAL OF CONTROLLED RELEASE, v.127, no.1, pp.41 - 49 JOURNAL OF CONTROLLED RELEASE 127 1 41 49 scie scopus 000255296200005 2-s2.0-40649100957 Chemistry, Multidisciplinary Pharmacology & Pharmacy Chemistry Pharmacology & Pharmacy Article SELF-ASSEMBLED NANOPARTICLES POLYMERIC MICELLES HPMA COPOLYMER DRUG-DELIVERY 5-BETA-CHOLANIC ACID CONTROLLED-RELEASE DOXORUBICIN CANCER CHEMOTHERAPY BIODISTRIBUTION hydrophobically modified glycol chitosan self-assembled nanoparticles cisplatin drug delivery system passive tumor targeting in vivo antitumor efficacy