Ju, Young Min Park, Kwideok Son, Jun Sik Kim, Jae-Jin Rhie, Jong-Won Han, Dong Keun 2024-01-20T23:32:19Z 2024-01-20T23:32:19Z 2021-09-03 2008-04 1552-4973 https://pubs.kist.re.kr/handle/201004/133590 Three dimensional (3D) porous poly(L-lactic acid) (PLLA) scaffolds were fabricated using a modified gas foaming method whose effervescent porogens were a mixture of sodium bicarbonate and citric acid. To improve chondrocyte adhesion, the scaffolds were then hydrophilized through oxygen plasma treatment and in situ graft polymerization of acrylic acid (AA). When the physical properties of AA-grafted scaffolds were examined, the porosity and pore size were 87 similar to 93% and 100 similar to 300 mu m, respectively. The pore sizes were highly dependent on the varying ratios (w/w) between porogen and polymer solution. Influenced by their pore sizes, the compressive moduli of scaffolds significantly decreased with increasing pore size. The altered surface characteristics were clearly reflected in the reduced water contact angles that meant a significant hydrophilization with the modified polymer surface. Electron spectroscopy for chemical analysis (ESCA) and time-of-flight secondary ion mass spectrometer (ToF-SIMS) also confirmed the altered surface chemistry. When chondrocytes were seeded onto the AA-grafted PLLA scaffolds, cell adhesion and proliferation were substantially improved as compared to the unmodified scaffolds. The benefit of the modified scaffolds was clear in the gene expressions of collagen type II that was significantly upregulated after 4-week culture. Safranin-O staining also identified greater glycosaminoglycan (GAG) deposition in the modified scaffold. The AA-grafted porous polymer scaffolds were effective for cell adhesion and differentiation, making them a suitable platform for tissue-engineered cartilage. (c) 2007 Wiley Periodicals, Inc. English WILEY INDUCED GRAFT-POLYMERIZATION SMOOTH-MUSCLE-CELLS SURFACE MODIFICATION ACRYLIC-ACID POLY(L-LACTIC ACID) POLY(GLYCOLIC ACID) GLOW-DISCHARGE FILMS PLASMA FABRICATION Beneficial effect of hydrophilized porous polymer scaffolds in tissue-engineered cartilage formation Article 10.1002/jbm.b.30943 1 JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, v.85B, no.1, pp.252 - 260 JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 85B 1 252 260 scie scopus 000254445400032 2-s2.0-41449106668 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article INDUCED GRAFT-POLYMERIZATION SMOOTH-MUSCLE-CELLS SURFACE MODIFICATION ACRYLIC-ACID POLY(L-LACTIC ACID) POLY(GLYCOLIC ACID) GLOW-DISCHARGE FILMS PLASMA FABRICATION tissue engineering porous polymer scaffolds gas foaming method plasma treatment hydrophilization chondrocyte