Shama, Naz Bai, Sang Wook Chung, Bong Chul Jung, Byung Hwa 2024-01-20T23:32:26Z 2024-01-20T23:32:26Z 2021-09-03 2008-04 0951-4198 https://pubs.kist.re.kr/handle/201004/133596 A quantitative analytical method using gas chromatography/mass spectrometry (GC/MS) to determine urinary concentrations of eight progesterones and corticosteroids has been developed. After enzymatic hydrolysis with beta-glucouronidase/arylsulfatase, urine samples were extracted by simple one-step solid-phase extraction. Obtained extracts were derivatized with a mixture of N-methyl-N-(trimethylsilyl)trifluoroacetamide/ammonium iodide/dithiothreitol and determined by GC/MS in selected ion monitoring mode to increase the sensitivity. d(4)-Cortisol and d(9)-progesterone were used as internal standards for two different steroid groups. The linear correlation coefficient was in the range of 0.9913 to 0.9998 and recoveries were over 80% for all compounds. Precision and accuracy were in the range of 0.9-18.1 and 84.1-118.7%, respectively. The limit of quantitation (LOQ) was 10 ng/mL for 11-deoxycorticosterone and 21-deoxycortisol and 5ng/mL for all other analytes. The developed method was successfully applied on pelvic organ prolapsed patients (n=10, age: 67.9 +/- 4.9) and post-menopausal (n=10, age: 63.6 +/- 5.5) control women. Urinary levels of most progesterones and corticosteroids except 11-deoxycorticosterone decreased but only that of 17 alpha-hydroxyprogesterone significantly decreased in patients compared with the control groups. Thus, it is concluded that progesterones could be a factor in the pathogenesis of pelvic organ prolapse, and, among them, 17 alpha-hydroxyprogesterone could be a biomarker for pelvic organ prolapse. Copyright (C) 2008 John Wiley & Sons, Ltd. English WILEY STEROID-HORMONE RECEPTORS SOLID-PHASE EXTRACTION INCONTINENCE ESTROGEN WOMEN LIGAMENTS THERAPY LC/MS GC/MS Quantitative determination of progesterones and corticosteroids in human urine using gas chromatography/mass spectrometry: application to pelvic organ prolapse patients Article 10.1002/rcm.3441 1 RAPID COMMUNICATIONS IN MASS SPECTROMETRY, v.22, no.7, pp.959 - 964 RAPID COMMUNICATIONS IN MASS SPECTROMETRY 22 7 959 964 scie scopus 000254759600008 2-s2.0-41549161611 Biochemical Research Methods Chemistry, Analytical Spectroscopy Biochemistry & Molecular Biology Chemistry Spectroscopy Article STEROID-HORMONE RECEPTORS SOLID-PHASE EXTRACTION INCONTINENCE ESTROGEN WOMEN LIGAMENTS THERAPY LC/MS GC/MS progesterones Corticosteroids GC-MS pelvic organ prolapse