Lee, Kyung Eun Kim, Ho Min Lee, Jie-Oh Jeon, Hyesung Han, Sung Sik 2024-01-20T23:33:38Z 2024-01-20T23:33:38Z 2021-08-31 2008-03-15 0927-7765 https://pubs.kist.re.kr/handle/201004/133653 The integral membrane protein CD40 was found on the surface of B lymphocytes that interact with CD40L on T cells during the immune response. The hydrophobic transmembrane domains of membrane proteins can be stabilized in detergent or in lipid bilayers such as liposomes. Membrane proteins can be incorporated into the liposome in a similar fashion to the way they are handled in vivo. In this study, a large amount of full-sequence CD40 was produced using a bacterial system that contained a Mistic construct. The CD40 was then reconstituted into liposomes by detergent-mediated reconstitution. All stages in the process of liposome disruption with various detergent ratios were easily observed by monitoring the optical density. The structure of the liposome and the reconstitution of CD40 were confirmed by cryo-TEM. The results of the present study show that the detergent ratio had an effect on the structure of the liposome and the amount of CD40 that was reconstituted into the liposome. (c) 2007 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV MEMBRANE-PROTEINS MICELLES Regulation of CD40 reconstitution into a liposome using different ratios of solubilized LDAO to lipids Article 10.1016/j.colsurfb.2007.09.021 1 COLLOIDS AND SURFACES B-BIOINTERFACES, v.62, no.1, pp.51 - 57 COLLOIDS AND SURFACES B-BIOINTERFACES 62 1 51 57 scie scopus 000255622800009 2-s2.0-38849167531 Biophysics Chemistry, Physical Materials Science, Biomaterials Biophysics Chemistry Materials Science Article MEMBRANE-PROTEINS MICELLES liposome reconstitution membrane protein CD40 LDAO solubilization Cryo-TEM