Jung, Youngmee Kim, Soo Hyun Kim, Sang-Heon Kim, Young Ha Xie, Jun Matsuda, Takehisa Min, Byoung Goo 2024-01-21T00:02:01Z 2024-01-21T00:02:01Z 2021-09-03 2008-01 0920-5063 https://pubs.kist.re.kr/handle/201004/133835 It is known that complex loading is involved in the development and maintenance of articular cartilage in the body. It means the compressive mechanical stimulation is a very important factor for formation of articular cartilage using a tissue-engineering technique. The objective of this study is to engineer cartilaginous constructs with mechano-active scaffolds and to evaluate the effect of dynamic compression for regeneration of cartilage. The mechano-active scaffolds were prepared from a very elastic poly(L-lactide-co-epsilon-caprolactone) (PLCL) with 85% porosity and 300-500 pm pore size using a gel-pressing method. The scaffold was seeded with 2 x 106 chondrocytes and the continuous compressive deformation of 5% strain was applied with 0.1 Hz for 10 days and 24 days, respectively. Then, the chondrocytes-seeded constructs were implanted subcutaneously into nude mice, Mechano-active scaffolds with complete rubber-like elasticity showed almost complete (over 97%) recovery at an applied strain of up to 500%. The amount of chondral extracellular matrix was increased significantly by mechanical stimulation on the highly elastic mechano-active scaffolds. Histological analysis showed the mechanically Stimulated implants formed mature and well-developed cartilaginous tissue, as evidenced by the chondrocytes within lacunae and the abundant accumulation of sulfated GAGs. However, unhealthy lacunae shapes and hypertrophy forms were observed in the implants stimulated mechanically for 24 days, compared with those stimulated for 10 days. In conclusion, the proper periodical application of dynamic compression can encourage chondrocytes to maintain their phenotypes and enhance the production of GAGs, which would improve the quality of cartilaginous tissue formed both it? vitro and in vivo. English TAYLOR & FRANCIS LTD ARTICULAR-CARTILAGE REPAIR CELLS Cartilaginous tissue formation using a mechano-active scaffold and dynamic compressive stimulation Article 10.1163/156856208783227712 1 JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, v.19, no.1, pp.61 - 74 JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION 19 1 61 74 scie scopus 000253227500005 2-s2.0-37849001743 Engineering, Biomedical Materials Science, Biomaterials Polymer Science Engineering Materials Science Polymer Science Article ARTICULAR-CARTILAGE REPAIR CELLS mechano-active tissue engineering elastic poly(L-lactide-co-epsilon-caprolactone) scaffold bioreactor compressive mode mechanical stimulation chondrocytes.