Islam, Nurul M. Yoo, Hye Hyun Lee, Min Woo Dong, Mi-Sook Park, Young In Jeong, Ha Sook Kim, Dong-Hyun 2024-01-21T00:02:25Z 2024-01-21T00:02:25Z 2021-09-02 2008-01 0958-0344 https://pubs.kist.re.kr/handle/201004/133854 A rapid and accurate reversed-phase liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method has been developed and validated for the quantitative determination of five flavonoid glycosides, icariin, epimedin A, epimedin B, epimedin C and hyperin in Herba Epimedii. Chromatographic separations were performed using a C-18 narrow-bore HPLC column; a mixture of an aqueous solution of ammonium formate (pH 4.0) and acetonitrile was used as the mobile phase, with compounds detected in the positive ion mode with multiple-reaction monitoring using a triple-quadrupole mass spectrometer equipped with an electrospray ionisation interface. This method for the determination of the reported flavonoid glycosides was accurate and reproducible, with a lower limit of quantification of 0.5 mu g/mL. The standard calibration curves for the above-mentioned compounds were linear (r(2) > 0.998) over the concentration range 0.5-10.0 mu g/mL. The relative standard deviations for intra- and inter-day precision over the concentration range for the flavonoid glycosides were lower than 7.8% with accuracy between 90.1 and 111.0%. The established method was successfully applied to the quality assessment of samples of Herba Epimedii collected from Korea and China. Copyright (C) 2007 John Wiley & Sons, Ltd. English WILEY CAPILLARY-ZONE-ELECTROPHORESIS DIODE-ARRAY DETECTION ICARIIN CONSTITUENTS EXTRACTS KOREANUM MS Simultaneous quantitation of five flavonoid glycosides in Herba Epimedii by high-performance liquid chromatography-tandem mass spectrometry Article 10.1002/pca.1018 1 PHYTOCHEMICAL ANALYSIS, v.19, no.1, pp.71 - 77 PHYTOCHEMICAL ANALYSIS 19 1 71 77 scie scopus 000253340200009 2-s2.0-39049132012 Biochemical Research Methods Plant Sciences Chemistry, Analytical Biochemistry & Molecular Biology Plant Sciences Chemistry Article CAPILLARY-ZONE-ELECTROPHORESIS DIODE-ARRAY DETECTION ICARIIN CONSTITUENTS EXTRACTS KOREANUM MS Herba Epimedii HPLC-MS/MS quantitative analysis flavonoid glycosides