Lee, Sang Kyu Jun, In Hye Yoo, Hye Hyun Kim, Ju Hyun Seo, Young Min Kang, Mi Jeong Lee, Seung Ho Jeong, Tae Cheon Kim, Dong Hyun 2024-01-21T00:02:35Z 2024-01-21T00:02:35Z 2021-08-31 2008-01 0951-4198 https://pubs.kist.re.kr/handle/201004/133862 The in vitro metabolism of deoxypodophyllotoxin (DPT), a medicinal herbal product isolated from Anthriscus sylvestris (Apiaceae), was investigated in rats and human microsomes and human recombinant cDNA-expressed CYPs. The incubation of DPT with pooled human microsomes in the presence of NADPH generated five metabolites while its incubation with dexamethasone (Dex)-induced rat liver resulted in seven metabolites (M1-M7) with major metabolic reactions including mono-hydroxylation, O-demethylation and demethylenation. Reasonable structures of the seven metabolites of DPT could be proposed, based on the electrospray tandem mass spectra. Chemical inhibition by ketoconazole and metabolism studies with human recombinant cDNA-expressed CYPs indicated that CYP 3A4 and 2C19 are the major CYP isozymes in the metabolism of DPT in human liver microsomes. Copyright (C) 2007 John Wiley & Sons, Ltd. English WILEY HUMAN CYTOCHROME-P450 3A4 CELL-CULTURES BIOCONVERSION INHIBITION ETOPOSIDE Characterization of in vitro metabolites of deoxypodophyllotoxin in human and rat liver microsomes using liquid chromatography/tandem mass spectrometry Article 10.1002/rcm.3325 1 RAPID COMMUNICATIONS IN MASS SPECTROMETRY, v.22, no.1, pp.52 - 58 RAPID COMMUNICATIONS IN MASS SPECTROMETRY 22 1 52 58 scie scopus 000252678100006 2-s2.0-38349083814 Biochemical Research Methods Chemistry, Analytical Spectroscopy Biochemistry & Molecular Biology Chemistry Spectroscopy Article HUMAN CYTOCHROME-P450 3A4 CELL-CULTURES BIOCONVERSION INHIBITION ETOPOSIDE