Chang, Jong Wook Kang, Un-Beom Kim, Dong Hyun Yi, Jae Kyo Lee, Jong Won Noh, Dong-Young Lee, Cheolju Yu, Myeong-Hee 2024-01-21T00:02:40Z 2024-01-21T00:02:40Z 2021-08-31 2008-01 1862-8346 https://pubs.kist.re.kr/handle/201004/133866 Comparative proteome analysis was performed on the cultured media of human nontumor and malignant breast cell lines, Hs578Bst and Hs578T, respectively, in search of a serological biomarker(s) for breast cancer. Proteins in the conditioned media were separated by 2-D PAGE and then visualized by silver-staining. Eight proteins changed differentially by more than twofold were identified by MALDI-TOF/TOF MS. Among the proteins identified, the terminal laminin-like globular (LG3) domain of endorepellin, which was recently reported as an antiangiogenesis factor, was decreased in the cancer cell line. We confirmed the bone morphogenic protein-1 (BMP-1) mediated cleavage site on the N-terminus of endorepellin LG3 fragment. This finding suggests that the LG3 fragment is specifically released by a BMP-1 driven limited proteolytic process. The protein was also detected in plasma by Western blot analysis and selected reaction monitoring (SRM). The plasma level of the endorepellin LG3 fragment was significantly lower in breast cancer patients compared to healthy donors (p = 0.017; n = 12). The LG3 protein concentration in the control plasma was measured at approximately 3.7 pmol/mL compared to 1.8 pmol/mL in plasma from the cancer patients. We suggest that these results support the potential use of the endorepellin LG3 fragment as a new serological biomarker for breast cancer. English WILEY-V C H VERLAG GMBH TISSUE GROWTH-FACTOR PROTEOMIC ANALYSIS IN-VIVO DISCOVERY PROTEINS PERLECAN MICROENVIRONMENT METASTASIS REVEALS MARKERS Identification of circulating endorepellin LG3 fragment: Potential use as a serological biomarker for breast cancer Article 10.1002/prca.200780049 1 PROTEOMICS CLINICAL APPLICATIONS, v.2, no.1, pp.23 - 32 PROTEOMICS CLINICAL APPLICATIONS 2 1 23 32 scie scopus 000252835500005 2-s2.0-38949128498 Biochemical Research Methods Biochemistry & Molecular Biology Biochemistry & Molecular Biology Article TISSUE GROWTH-FACTOR PROTEOMIC ANALYSIS IN-VIVO DISCOVERY PROTEINS PERLECAN MICROENVIRONMENT METASTASIS REVEALS MARKERS biomarker breast cancer endorepellin LG3 fragment selected reaction monitoring