Kim, Youn-Jung Song, Mi-Kyung Mee-Song Ryu, Jae-Chun 2024-01-21T00:02:47Z 2024-01-21T00:02:47Z 2021-09-02 2007-12-31 1738-642X https://pubs.kist.re.kr/handle/201004/133871 Exposure to DNA-damaging agents can elicit a variety of stress-related responses that may alter the expression of genes associated with numerous biological pathways. We used 19 k whole human genome chip to detect gene expression profiles and potential signature genes in human normal hepatocytes (THLE-3) by treatment of five direct acting mutagens, furylfuramide (AF-2), N-nitroso-N-methylurea (MNU), methylmethanesulfonate (MMS), 4-nitroquinoline-N-oxide (4-NQO) and 2-nitrofluorene (2NF) of the IC20 concentration for 3 h. Fifty one up-regulated common genes and 45 down-regulated common genes above 1.5-fold by five direct-acting mutagens were identified by clustering analysis. Many of these changed genes have some association with apoptosis, control of cell cycle, regulation of transcription and signal transduction. Genes related to these functions, as TP73L, E2F5, MST016, SOX5, MAFB, LIF, SII3, TRIS, EMR1, CYTL1, CX3CR1 and RHOH are up-regulated. Down-regulated genes are ALOX15B, xs155, IFITM1, BATF, VAV2, CD79A, DCDC2, TNFSF8 and KOX8. We suggest that gene expression profiling on mutagens by toxicogenomic analysis affords promising opportunities to reveal potential new mechanistic markers of genotoxicity. English KOREAN SOCIETY TOXICOGENOMICS & TOXICOPROTEOMICS-KSTT EXPRESSION PROFILES GENE-EXPRESSION CANCER TUMORS CELLS Toxicogenomic study to identify potential new mechanistic markers on direct-acting mutagens in human hepatocytes (THLE-3) Article 1 MOLECULAR & CELLULAR TOXICOLOGY, v.3, no.4, pp.231 - 237 MOLECULAR & CELLULAR TOXICOLOGY 3 4 231 237 scie kci other 000252045500003 Biochemistry & Molecular Biology Toxicology Biochemistry & Molecular Biology Toxicology Article EXPRESSION PROFILES GENE-EXPRESSION CANCER TUMORS CELLS mutagen DNA damage toxicogenomics gene expression analysis THLE-3 cell